中文摘要：

探讨了铜绿假单胞菌粗酶液对十溴联苯醚（BDE-209）的降解特性,结果表明,该菌株胞内酶在12 h内对1 mg.L-1的BDE-209降解率达到了69.22%,温度、pH值、酶蛋白浓度及底物BDE-209浓度均会影响胞内酶粗提液对BDE-209的降解效率.当BDE-209浓度为1 mg.L-1时,BDE-209适宜的酶促降解条件为：温度30℃,反应pH值7.5,且降解率随着酶浓度的增加而增大.胞内酶对BDE-209的降解过程符合一级动力学反应模型,底物浓度为1 mg.L-1时降解速率最快,半衰期为6.9 h.胞内酶降解BDE-209符合高浓度底物抑制的酶促反应类型,其基本降解动力学参数为rmax=0.133 mg.（L.h）-1,Km=0.642mg.L-1,Ki=1.558 mg.L-1.

英文摘要：

The degradation characteristics of decabromodiphenyl ether（BDE-209） by crude enzyme from Pseudomonas aeruginosa were investigated.The results revealed that the degradation efficiency of the intracellular enzyme excreted from this bacterial strain reached 69.22% after incubation with 1 mg·L-1 BDE-209 for 12 h.Temperature,pH,enzyme concentration and BDE-209 concentration all influenced the ability of crude enzyme to degrade BDE-209.When the BDE-209 concentration was 1 mg·L-1,the optimal condition for enzymatic degradation was temperature 30℃ and pH 7.5,and the degradation rate increased with increasing enzyme concentration.The degradation process of BDE-209 by intracellular enzyme of the strain conformed to the first-order kinetic model.The highest reaction rate was achieved when the initial concentration of BDE-209 was 1 mg·L-1 and the half-life of this substrate was 6.9 h.In addition,the biodegradation of BDE-209 can be well described by enzymatic reaction of high concentration substrate inhibition,with a maximum substrate utilization rate of 0.133 mg·（L·h）-1,a Michaelis-Menten constant of 0.642 mg·L-1,and an inhibitory constant of 1.558 mg·L-1,respectively.