中文摘要：

目的考察苦参碱（matrine，Mat）对皮肤角质形成细胞（keratinocytes，Kc）系HaCaT细胞白细胞介素-17受体（IL-17R）、p—p38 MAPK表达的影响，及其对Hacarr细胞分泌白细胞介素（IL）-6、IL-8、MIP-3α等炎症因子的影响。方法采用RT-PCR方法检测Mat对皮肤KCIL-17R mRNA表达的影响；采用流式细胞术检测Mat对HaCaT细胞IL-17R蛋白含量的影响；采用Western blot方法检测Mat对HaCaT细胞表达p-p38 MAPK的影响；ELISA法检测Mat对HaCaT细胞分泌炎症因子IL-6、IL-8及MIP-3理的影响。结果Mat可降低Hacarr细胞表达IL-17R和p-p38 MAPK，以及降低Hac打细胞IL-6、IL-8、MIP-3仪等炎症因子的分泌。结论Mat可通过抑制IL-17R的表达，阻止p38 MAPK的磷酸化过程而具有抗炎作用。

英文摘要：

OBJECTIVE To investigate the effect of matrine on the expression of IL-17R, p-p38 MAPK in skin keratinocyte cell line HaCaT cells, and on the level of IL-6, IL-8, MIP-3α secreted by HaCaT cells. METHODS RT-PCR method was applied to analyze the expression of IL-17R mRNA in HaCaT cells treated with matrine. Flow cytometry was used to analyze the IL-17R protein content. The expression of p-p38 MAPK was determined by Western blot method. The levels of inflammatory factors IL-6, IL-8 and MIP- 3α were analyzed by ELISA. RESULTS Matrine decreased the expression of IL-17R and p-p38 MAPK in HaCaT cells, and the level of IL-6, IL-8 and MIP-3α secreted by HaCaT cells. CONCLUSION Matrine can inhibit the expression of IL-17R to prevent the phosphorylation of p38 MAPK, and inhibit the expression of IL-6, IL-8, MIP-3α, and so matrine has anti-inflammatory effect.