中文摘要：

目的建立黄柏碱与木兰花碱的色谱分离方法，并通过考察各影响因素，探讨离子液体在分离过程中的作用机制。方法以离子液体1-丁基-3-甲基咪唑四氟硼酸盐为流动相添加剂，用高效液相色谱法分离黄柏碱和木兰花碱，并分别考察流动相pH、离子强度、缓冲溶液种类、离子液体的种类、柱温等影响因素，探讨离子液体在整个分离过程中的作用机制。结果以20mmol·L-1离子液体作为流动相添加剂等度洗脱即能达到两种生物碱的基线分离。两组分的保留值、分离度及理论板数随着流动相条件的改变而不同。结论离子液体在黄柏碱与木兰花碱的分离过程中以生成中性离子对的作用为主。

英文摘要：

Objective The chromatographic separation of phellodendrine and magnoflorine was established, and the mechanism of ionic liquid in the process was studied by investigating influencing factors. Methods Phellodendrine and magnoflorine were isolated by HPLC with 1-butyl-3 -methyl imidazole four fluorine boric acid as a mobile phase additive, and mobile phase pH, ionic strength, buffer solution, ionic liquids and column temperature were all studied by investigating the effect of ionic liquid in the process. Results It was shown that the analytes were separated at baseline with a mobile phase containing ionic liquid under 20 mmol ~ L-~ isocratic elution. The retention value, separation and number of theoretical plates varied with the change of mobile phase. Conclusion It is clarified thai ionic liquid mainly acts as neutral ion pair in the separation of phellodendrine and magnoflorine.