中文摘要：

目的：探讨HPV-16 E6和E7癌蛋白对肺癌细胞A549迁移和侵袭能力的影响及其机制。方法：A549细胞分别转染含HPV-16 E6、E7的EGFP质粒,采用划痕实验观察转染后不同时间的迁移能力,Transwell侵袭实验检测侵袭能力,Western blotting和实时定量PCR分别分析MMP-2和MMP-9的蛋白、mRNA表达。结果：同对照细胞相比,转染含HPV-16 E6、E7质粒的细胞迁移能力增强,在36h和48h时趋势较为明显;转染HPV-16 E6、E7组的侵袭细胞数分别为每高倍视野121.3±13.3、129.7±5.5,其显著高于对照组（P〈0.01）。而且,转染HPV-16 E6、E7的细胞中MMP-2和MMP-9的蛋白和mRNA表达显著增加（P〈0.01）。结论：HPV-16 E6和E7癌蛋白可增强A5 4 9细胞的迁移和侵袭能力,其机制与上调MMP-2和MMP-9表达有关。

英文摘要：

Objective：To study the effects of human papillomavirus （HPV）type 16 （HPV -16）oncoproteins on migration and invasion in A549 lung cancer cells and the underlying mechanisms.Methods：A549 cells were trans-fected with EGFP plasmids harboring HPV -16 E6 and E7 genes,respectively.After transfection,migration capability at different time was analyzed by wound healing assay.Invasion capability was detected by transwell invasion assay. The expression of MMP -2 and MMP -9 protein and mRNA was determined by Western blotting and real -time PCR,respectively.Results：Compared with control cells,the migration capability in the cells transfected with the plas-mids harboring HPV -16 E6 or E7 was enhanced,which was more obvious at 36h and 48h.The invasive cell number in the cells transfected with the plasmids harboring HPV -16 E6 and E7 was 121.3 ±13.3 and 129.7 ±5.5,respec-tively,which was much higher than that in control cells （P 〈0.01）.Furthermore,the expression of MMP -2 and MMP -9 protein and mRNA in the cells transfected with the plasmids harboring HPV -16 E6 or E7 was dramatically upregulated（P 〈0.01）.Conclusion：HPV -16 E6 and E7 oncoproteins can promote the capabilities of migration and invasion,which may be related to the upregulation of MMP -2 and MMP -9 expression.