中文摘要：

目的：探讨4条登革病毒抗原肽在不同遗传背景小鼠中的免疫原性。方法：4条登革病毒抗原肽（C45-57KLV-MAFIAFLRFL,E396-408SSIGKMFEATARG,NS323-35YRILQRGLLGRSQ和NS3141-155NREGKIVGLYGNGVV）中每条肽分别免疫BALB/c小鼠和C57BL/6小鼠;3周后,处死小鼠并制备脾细胞悬液;不刺激或同样抗原肽刺激脾细胞后,采用细胞内细胞因子染色流式细胞术（ICS）检测小鼠脾细胞CD4^＋ T细胞中肽特异性产生IFN-γ或IL-4的CD4^＋ T细胞的百分比。结果：肽C45-57可诱导BALB/c小鼠产生特异性的IFN-γ^＋ CD4^＋ T细胞（0.72%±0.04% vs 0.04%±0.02%,P〈0.05）而肽E396-408则诱导产生特异性的IL-4^＋ CD4^＋ T细胞（0.09%±0.01%vs0.01%±0.01%,P〈0.05）;肽E396-408、NS323-35和NS3141-155均可诱导C57BL/6小鼠产生特异性的IFN-γ^＋ CD4^＋ T细胞（分别为0.31%±0.03%vs0.02%±0.01%,P〈0.05;0.21%±0.03% vs 0.04%±0.01%,P〈0.05;0.44%±0.04% vs 0.02%±0.01%,P〈0.05）,而肽C45-57可诱导产生特异性的IL-4^＋ CD4^＋ T细胞（0.45%±0.05% vs 0.02%±0.02%,P〈0.05）。结论：肽C45-57和E396-408在BALB/c小鼠中具有免疫原性而肽C45-57、E396-408、NS323-35和NS3141-155在C57BL/6小鼠中具有免疫原性。

英文摘要：

Objective：To explore the immunogenecity of four Dengue virus-specific peptides in mice. Methods： Each peptide of four Dengue virus-specific peptides （ C45-57 KLVMAFIAFLRFL, E396-408 SSIGKMFEATARG, NS323-35 YRILQRGLLGRSQ, and NS3141-155 NREGKIVG- LYGNGW） was used to immunize BALB/c mice and C57B1_./6 mice,respectively. Three weeks later, mice were killed and splelocytes were prepared. Splelocytes were stimulated by the same peptide and intracellular cytokine staining （ICS） assay was used to detect the percentages of peptide-specific IFN-γ or IL-4 producing CD4^＋ T cells in total CD4^＋ T cells. Results： Peptide C45-57 induced peptide-specific IFN-γ^＋ CD4^＋ T cells （0.72 % ±0.04 % vs 0.04 % ± 0.02 %, P 〈 0.05 ） while peptide E396-408 induced peptide- specific IL-4^＋ CD4^＋ T cells （0.09 % ± 0.01% vs 0.01% ± 0.01%, P 〈 0.05 ）in BALB/c mice; Peptide E396.408, NS323.35 and NS3141-155 induced peptide-specific IFN-y＋ CD4 ＋ T ceils （0.31% ±0.03% vs 0.02%± 0.01%, P 〈 0.05;0.21% ±0.03% vs 0.04% ± 0.01%, P 〈 0.05;0.44% ± 0.04% vs 0.02%± 0.01%, P 〈 0.05,respectively） while peptide C45-57 induced peptide-specific IL-4^＋ CD4^＋ T cells （0.45% ± 0.05% vs 0.02% ± 0.02%, P 〈 0.05）in C57BL/6 mice. Conclusion： Peptide C45-57 and E396-4os have immunogenecity in BALB/c mice while peptide C45-57, E396-408, NS323-35 and NS3141-155 have immunogenecity in C57BL/6 mice.