中文摘要：

目的：研究前期实验鉴定的2条丙型肝炎病毒HLA-A＊0201限制性CTL（活化的CD8＋T细胞）表位的体内外免疫学效应。方法：采用丙型肝炎病毒HLA-A＊0201限制性CTL表位肽C_181（LLSCLTTPV）和NS2_172（VLQAGLIRV）分别体外刺激HLA-A＊0201阳性健康志愿者外周血单个核细胞（PBMCs）和免疫HLA-A＊0201转基因小鼠,采用酶联免疫斑点实验（ELISPOT）和细胞内细胞因子染色（ICS）/流式细胞术检测表位肽特异性CD8＋T细胞的水平。结果：C_181和NS2_172诱导HLA-A＊0201阳性PBMCs产生了高水平的肽特异性分泌IFN-γ的细胞和肽特异性IFN-γ＋CD8＋T细胞;在C_181和NS2_172免疫的HLA-A＊0201转基因小鼠脾细胞中检测到了肽特异性分泌IFN-γ的细胞和肽特异性IFN-γ＋CD8＋T细胞。结论：C_181和NS2_172具有较好免疫学效应,有望用于研发CTL表位肽疫苗。

英文摘要：

Objective： To explore the immunogenecity of two previously identified hepatitis C virus-derived HLA-A＊0201-restricted CTL epitopes.Methods： Hepatitis C virus-specific CTL epitopes C_181（LLSCLTTPV） and NS2_172（VLQAGLIRV） were utilized to stimulate peripheral blood mononuclear cells（PBMCs） isolated from HLA-A＊0201-positive healthy volun-teers in vitro and immunize HLA-A＊0201 transgenic mice,respectively.Enzyme-linked immunospot assay（ELISPOT） and intracellular cytokine staining（ICS）/flow cytometry were applied to detect CTL epitopes-specifc CD8＋ T cells（CTL）.Results： Among peptide-stimulated PBMCs,there were high levels of peptide-specific CTL directed to C_181 and NS2_172.In addition,C_181 and NS2_172-specific CTL were induced in splenocytes from peptide-vaccined HLA-A＊0201 transgenic mice.Conclusion： C_181 and NS2_172 are capable of inducing CTL response in vivo and in vitro,and are useful for the development of hepatitis C virus CTL epitopes-based vaccine.