中文摘要：

利用Langmuir-Blodgett（LB）技术研究了纤维蛋白I-型胶原蛋白在去离子水亚相、氯化钙亚相和碳酸氢钙亚相上的成膜行为和形貌．表面压．面积等温（π-A）曲线表明纤维蛋白I-型胶原蛋白在三种亚相上均具有较好的成膜性能，利用原子力显微镜（AFM）对转移到新鲜解理的云母表面的单层I-型胶原蛋白的形貌进行了分析，AFM结果表明胶原蛋白在界面聚集成多个胶原分子组成的束装．该研究结果对于理解胶原在气．液界面的成膜性能具有重要意义，同时对探究纤维蛋白I-型胶原蛋白调节下的生物矿化作用具有重要的指导意义．

英文摘要：

Spread monolayers of the fibril protein I-collagen were studied at the air - water interface in the presence of water, CaC12 solution and calcium bicarbonate solution. The surface pressure isotherms curve （π- curve） of collagen monolayers showes that collagen has a good ability to spread monolayers at air-water. The morphology of collagen LB films has been characterized by atom force microscopy （AFM） , and the results in- dicate that spread collagen monolayers at the airwater interface have the ability to form supramolecular struc- tures （fibrils）. It is very important to understand its ability for spreading monolayers; meantime, it has poten- tial value to study the role of the collagen played in the process of biomineralization.