中文摘要：

目的：检测酸敏感离子通道（ASICs）在大鼠破骨细胞中的表达情况，探讨其在破骨细胞中的作用。方法：采用1，25-（OH）2D3诱导大鼠骨髓单核细胞分化的破骨细胞。RT-PCR扩增ASICs基因；免疫印迹检测ASICs转录水平；免疫荧光细胞化学方法观察ASICs基因在破骨细胞中的表达和分布。结果：诱导5d后可见抗酒石酸盐酸性磷酸酶（TRAP）阳性多核细胞出现，大鼠破骨细胞在mRNA和蛋白水平均可检测到ASIC1、ASIC2和ASIC3基因的表达产物。免疫荧光细胞化学显示ASIC1和ASIC2蛋白在细胞膜中有较强的表达，而ASIC3主要定位于细胞质中，少量定位于细胞膜上。结论：大鼠骨髓单核细胞可诱导分化成破骨细胞，能表达ASICs。

英文摘要：

Objective： To determine the expression of acid-sensing ion channels （ASICs） gene in rat osteoclasts to explore the possible role of ASICs in osteoclasts. Methods： Osteoclasts were induced by bone marrow mononuclear cells of rat using medium with 1, 25-（OH）2D3. RTPCR and Western blot were usel to analyse ASICs in rat osteoclasts. The distribution of ASICs in osteoclasts was detected by indirect immunofluoreseence staining. Results： The TRAP positive multinuclear cells were induced from rat marrow mononuclear ceils after inoculating for 5 days. RT-PCR analysis showed that ASIC1, ASIC2 and ASIC3 mRNA were present in the osteoclasts, and the corresponding proteins expressions were detected. ASIC1 and ASIC2 were distributed along the cell membrane. The immunosignal of ASIC3 was even stronger in the cytoplasmic fraction than that on the membrane. Conclusion： Rat bone marrow mononuclear cells could be induced to differentiate into osteoclasts which express ASICs.