中文摘要：

目的评价细胞实验时异氟醚鼓泡给药法的可靠性。方法取直径8．6cm的大培养皿100个，每个大培养皿内置3个直径3．5cm用于细胞培养的小培养皿，大培养皿置于37屯、5％CO2培养箱中。使用ABV—A麻醉机给药，氧气：氮气比为1：4，混合异氟醚后（鼓泡气），经人工鼻过滤，接21G针头置于大培养皿中央底部持续鼓泡，气体流量与培养液容积比例为0．25L／min：80ml。按鼓泡气异氟醚浓度将大培养皿随机分为10组（n=10），Ⅰ组-Ⅷ组鼓泡气异氟醚浓度分别为0．2％、0．4％、0．8％、1．6％、2．0％、2．4％、3．2％和4．0％；Ⅸ组鼓泡气内未混合异氟醚；Ⅹ组未通人气体。Ⅰ组～Ⅷ组分别于鼓泡通气5、10、15、30、60和120min时（T1-6）于小培养皿的底部取细胞培养液7ml，采用气相色谱法测定异氟醚浓度。结果与T3时比较，Ⅰ组～Ⅷ组T1，2时异氟醚浓度降低（P〈0．05），T4-6时差异无统计学意义（P〉0．05）；异氟醚浓度（Y）与鼓泡气异氟醚浓度（X）直线回归方程为Y=1．5652X-0．1252。结论细胞实验时鼓泡给药法可提供稳定、可靠的异氟醚浓度。

英文摘要：

Objective To investigate the reliability of bubbling method for delivering isoflurane during cell experiment in vitro. Methods A Petri dish 8.6 cm in diameter, which contained 3 small Petri dishes 3.5 cm in diameter used for cell culture, was placed in a 5 % CO2 incubator at 37 ℃ . Liquid culture medium 80 ml composed of DMEM and F12 （ratio 1：1） and 10% fetal calf serum was infused into the big dish and overwhelmed the small dishes. A 21 G needle was inserted into the culture medium in the middle of big dish. A stream of gas mixture composed of 20% 02, 80% N2 and different concentrations of isoflurane was continuously bubbled into the culture media at 0.25 L/min. The liquid culture media were divided into 10 groups according to isoflurane concentration in the air stream ： in group Ⅰ～Ⅷ the concentrations of isoflurane in the air stream were 0.2 %, 0.4 %, 0.8 %, 1.6% , 2.0%, 2.4% , 3.2% and 4.0% respectively; in group Ⅸ there was no isoflurane in the air stream composed of only 20% O2 and 80% N2 and in group Ⅹ no air stream bubbled in the culture medium. The culture mediawere collected after the air stream had bubbuled in it for 5, 10, 15, 30, 60, 120 and 180 min for measurement of isoflurane concentration by double balanced headspace gas chromatography. Results The isoflurane concentration achieved balance between the bubbling air and culture medium after 15 min bubbling. Conclusion Bubbling method can provide stable and reliable concentration of isollurane in the liquid culture medium during cell experiment in vitro.