中文摘要：

用1-丁基-3-甲基咪唑六氟磷酸盐（[BMIM]PF6）疏水性离子液体作修饰剂制作[BMIM]PF6-修饰玻碳电极。在0.2mol/L磷酸盐缓冲液（pH7.0）中,运用差示脉冲溶出伏安法（DPSV）研究木犀草素在修饰电极上的电化学行为,建立测定金银花中总黄酮含量的新方法。研究表明,该修饰电极降低了木犀草素的氧化还原峰电位,增大了其氧化还原反应的峰电流。木犀草素氧化峰电流与其浓度在1.0×10^-10～1.6×10^-8mol/L范围内呈良好的线性关系,检出限达到3.2×10^-11mol/L,回收率为98.7%～103.6%。该法操作简单、快速、灵敏、准确,可用于金银花中总黄酮的测定。

英文摘要：

A glassy carbon electrode modified with hydrophobicity 1-n-butyl-3-methylimidazolium hexafluorophosphate （[BMIM]PF6/GCE） was fabricated. Differential pulse stripping voltammetry was used to investigate the electrochemical behavior of luteolin at the modified electrode in 0.2 mol/L phosphate buffer solution, pH 7.0 and a new method for determining total flavonoids in Flos Ionicera was established. It was found that the peak potentials for oxidation and reduction both shifted negatively by 15 mV at the modified electrode as compared with the corresponding bare electrode in 0.2 mol/L phosphate buffer solution （pH 7.0） containing 3 × 10-9 mol/L luteolin and higher oxidation and reduction peak currents of luteolin at the modified electrode were observed. Further experiments demonstrated that oxidation peak current increased linearly with increasing luteolin concen-tration within the range from 1.0 × 10-10 to 1.6 × 10-8 mol/L with a detection limit of 3.2 × 10-11 mol/L and the range of spike recovery at four levels was between 98.7% and 103.6%. The method is simple, fast, sensitive and accurate, thereby providing an available approach for the determination of total flavonoids in Flos Ionicera.