中文摘要：

通过免疫组织化学法,应用CD3、CD4和CD8单克隆抗体研究了CD3＋T淋巴细胞及CD4＋和CD8＋T淋巴细胞亚群在鸡盲肠扁桃体中的出现、迁移、组织定位分布及数量变化规律等一系列发育过程。结果显示：CD3＋、CD8＋T淋巴细胞最初于11胚龄时出现,而CD4＋T淋巴细胞在15胚龄时出现。在定位分布变化上,CD3＋主要分布在黏膜上皮中,CD4＋主要分布在黏膜固有层中,CD8＋在黏膜固有层和黏膜上皮内都有大量分布。在数量变化上,1～7日龄雏鸡各种阳性细胞的数量都骤然增加;而到7日龄时,雏鸡CD3＋淋巴细胞的数量明显减少,CD4＋、CD8＋T细胞的数量无明显变化;从21日龄开始直到35日龄,雏鸡CD3＋、CD4＋和CD8＋T淋巴细胞的数量持续增加。试验证明,鸡在出壳后初期十二指肠的细胞免疫功能增强。

英文摘要：

By immunohistochemistry,the CD3 ＋ T lymphocytes and CD4 ＋ and CD8 ＋ T cell subsets in chicken cecal tonsil,including appearance,migration,tissue localization and changes of the number,were determined by applying monoclonal antibodies of CD3,CD4 and CD8.The results showed that CD3 ＋,CD8 ＋ T lymphocytes first appeared on 11 day-old embryo,while CD4 ＋ T lymphocytes in the 15th day.Changes in distribution,CD3 ＋ cell smainly in the mucosal epithelium,CD4 ＋ cells in the mucosa in the lamina propria,but CD8 ＋ cells in the lamina propria and mucosal epithelium.Changes in the amounts,the number of positive cells were all increased dramatically before the 7th day,and at the 7th day,CD3 ＋ cells significantly decreased in the number,but CD4 ＋,CD8 ＋ T cells had no significant changes in the number.From the 21st day to the 35th day,CD3 ＋,CD4 ＋ and CD8 ＋ T lymphocytes in the number increase continuously.The conclusion could be made that the immune function can be enhanced in the early post-hatched chicken duodenum.