中文摘要：

应用IgM、IgG和IgA单克隆抗体的免疫组织化学方法,研究IgM^＋、IgG^＋和IgA^＋B淋巴细胞在鸡脾脏中的出现、迁移、数量变化规律等一系列的发育过程以及组织定位分布特点。结果显示,IgM^＋和IgA^＋细胞在胚胎15日龄时开始出现,IgG^＋细胞在胚胎18日龄时出现,21日龄雏鸡脾脏的红髓和生发中心中出现以IgG型为主的浆细胞。各日龄IgM^＋、IgG^＋和IgA^＋细胞主要分布在脾脏的特征性组织结构—椭球周围淋巴鞘和生发中心中,这些结构也随着B淋巴细胞的增多而不断发育成熟。B淋巴细胞的数量随着日龄增长持续增加,直至21日龄时趋于稳定,各日龄B淋巴细胞数量始终以IgG^＋细胞最多,IgM^＋细胞次之,IgA^＋细胞最少。结果表明,鸡出壳初期,脾脏的体液免疫功能逐渐增强,并在21日龄时达到成熟水平。

英文摘要：

The tissue localization and a series of development processes,such as appearance,migration,and quantity change in chicken spleen was immunohistochemically conducted by using the IgM,IgG and IgA monoclonal antibodies.The findings showed that IgM^＋ and IgA^＋ cells first appeared in caecal tonsil at embryonic day 15,IgG^＋ cells at embryonic day 18,and plasma cells in red pulp and germinal center at 21-day-old.For each age,IgM^＋,IgG^＋ and IgA^＋ cells were mainly distributed in characteristic structure,such as periellipsoid lymphatic sheath （PELS） and germinal center,gradually matured with increase of B lymphocytes.B lymphocytes kept increasing with the increase of age,and stable by 21-day-old,the number of IgG^＋ cells were most,IgM^＋ cells were less,and IgA^＋ cells were least all the time.The results reveal that the humoral immune function of spleen in chicken strengthen gradually after hatching,and reaches the mature level at 21 day-old.