为了解鸡在胚胎期以及出壳后的机体免疫状态,本研究采用SYBRGreenI实时荧光定量RT-PCR方法,检测鸡在胚胎时期和出壳后(1日龄~35日龄)脾脏中IFN-γ和IL-2mRNA表达水平,并研究鸡脾脏免疫功能的建立情况。研究发现:鸡脾脏中IFN-γ和IL-2的基因表达最早于13胚龄被检测到,随后迅速升高,分别于出壳后7日龄和10日龄达到峰值。出壳后,鸡脾脏中同一种细胞因子的基因表达水平显著高于胚胎时期。研究还表明:IFN-γ和IL-2的基因表达模型与出壳后鸡脾脏的发育以及T细胞的迁移定殖情况相一致。该研究对从分子水平,进一步了解鸡在胚胎时期和出壳后,脾脏免疫功能的建立情况具有重要意义。
In the present study, the ontogeny of the chicken spleen immune function was investigated by quantitative analysis of chicken IFN-γ and IL-2 mRNA in the chicken spleen during embryogenesis and the posthatch period (day 1 to day 35) using SYBR Green I based real-time RT-PCR method. The results showed that expression of IFN-γ and IL-2 genes in the spleen became detectable as early as 13 day old embryo, then increased gradually and peaked by 7 days and 10 days posthatch respectively. Expression of cytokine genes were also higher in the spleen of posthatch chickens compared with chick embryos. This expression pattern was in accordance with the completion of T-cell colonization and structural development of the spleen during the posthatch period.