中文摘要：

目的探讨Bryostatin-1对树突状细胞（DC）免疫功能的调节作用。方法联合应用GM-CSF和IL-4自人外周血单核细胞定向分化DC;以Bryostatin-1刺激TNF-α诱导成熟的DC,收集DC及其上清夜,以FACS和ELISA方法分析DC表面免疫分子（CD80、CD83、CD86、HLA-DR和B7-H1）和细胞因子（IFN-γ、IL-1β、IL-10和IL-12）表达水平;自DC提取RNA,以Northern blot分析DC的B7-h1 mRNA表达水平;以DC为诱导细胞,进一步检测DC的免疫诱导功能。结果Bryostatin-1通过降低DC表面B7-H1表达和增强B7.2表达,以及调节DC细胞因子（IL-1b、IL-12和IFN-γ）分泌,增强DC的免疫诱导功能,PKC通道特异性的抑制剂BI明显逆转Bryostatin-1的上述作用。结论Bryostatin-1增强DC免疫功能,其机制可能是激活PKC通道。

英文摘要：

In this study,we aimed to explore if Bryostatin-1 modulates the immune function of dendritic cells（DC）.DC were prepared from monocyte of peripheral blood by using GM-CSF and IL-4,and then stimulated DC with Bryostatin-1;by using FACS and ELISA,we tested the co-stimulators（CD80,CD83,CD86,HLA-DR,and B7-H1） expression and the cytokines（IFN-γ,IL-1β, IL-10,and IL-12） secretion of the stimulated DC.Then we extracted total RNA from DC and examined B7-H1 mRNA expression with Northern blot,as well as examined the immune function of DC to induce proliferation of T lymphocyte with mixed lymphocyte reaction. We found that Bryostatin-1 could enhance the immune function of DC by down-regulating B7-H1,up-regulating B7.2 expression on DC,and modulating cytokine（IL-1b,IL-12,and IFN-γ） production by DC.But Bisindolylmaleimide（BI）,a specific PKC inhibitor, abolished the regulation of Bryostatin-1 on B7-H1 and B7.2 expression.We also found that blockade of B7-H1 on DC could enhanced the ability of DC to induce allogeneic T lymphocyte proliferation.Our results demonstrate that Bryostatin-1 can enhance immune function of host by improving the immunity of DC,which indicates that Bryostatin-1 may play more important role on initiation and modulation immune response against tumor and infectious disease through this new mechanism.The possible mechanism is Bryostatin-1 activates PKC.