中文摘要：

目的研究神经肽VIP能否调节LPS激活的树突状细胞(DC)的免疫功能。方法联合应用rm GM-CSF和rmIL-4自C57BL/10.A小鼠骨髓细胞制备DC;以LPS和/或VIP刺激DC;收集DC及其上清夜行ELISA分析;自DC提取总RNA行RNAse分析。结果VIP明显抑制LPS激活的DC分泌细胞因子IL-2、IL-12和TNF-α,但对IL-6的抑制作用则不明显;VIP明显抑制LPS激活的DC表达化学因子MIP-2和-10,但对化学因子Rantes、MIP-1α和MIP-1β的抑制作用并不明显。结论VIP对LPS激活的DC的免疫功能有负性调节作用。

英文摘要：

Objective To investigate the modulating effects of vasoactive intestinal peptide(VIP) on the immune function of LPS-stimulated dendritic cell.Methods DCs were prepared from bone marrow of C57BL/10.A with rmGM-CSF and rmIL-4,and then stimulated with different stimuli for different times.The stimulated DCs and supernatant were collected for ELISA analysis;total RNA was extracted from the stimulated DC and analyzed by RNAse protection Assay.Results VIP significantly inhibited LPS-stimulated DC to produce IL-2,...