中文摘要：

目的：克隆并表达艰难梭菌毒素A羧基末端受体结合区（CDTAR）基因．方法：PCR扩增CDTAR基因并将其克隆到表达载体pET-22b（＋），重组质粒转化到E．coli BL21（DE3），经IPTG诱导表达，聚丙烯酰胺凝胶电泳（SDS—PAGE）对表达产物进行分析．结果：构建了含CDTAR基因的重组质粒pET-CDTAR，IPTG诱导后SDS-PAGE显示表达出M，约为35．7ku的重组蛋白，占菌体总蛋白的36．1％，可溶性表达占上清的22．2％，包涵体中约占24．9％．结论：成功克隆了CDTAR基因，并构建表达了CDTAR重组蛋白，为进一步研究CDTAR功能及研制艰难梭菌疫苗奠定了基础。

英文摘要：

AIM： To obtain the high expression of the gene coding for clostridium difficile toxin A receptor binding zone （CDTAR）. METHODS： The clostridium difficile toxin A Cterminal repeated gene was amplified by PCR and cloned into the prokaryotic expression vector pET-22b（ ＋ ） , and the recombined plasmid pET-CDTAR was transformed into E. coli strain BL21 （DE3）. The recombined vector was confirmed by digestion with EcoRI/XhoI and sequencing. The E. coli strain BL21 （DE3） containing pET-CDTAR was induced with IPTG and analyzed with SDS-PAGE. RESULTS： A 35.7 ku protein was acquired after inducing with IPTG and thin layer scanning suggested that CDTAR occupied 36.1% of the total bacterial protein, 22.2% of the supematant and 24.9% of the inclusion body. CONCLUSION： The cloning and high expression of clostridium difficile toxin A receptor gene lay a foundation for the further study on CDTAR function and clostridium difficile vaccine.