中文摘要：

目的：观察重度脓毒症小鼠吸入氢气后的存活情况及其血清高迁移率族蛋白B1（highmobility group box1,HMGB1）的水平,探讨氢气对脓毒症动物器官功能保护作用的分子学机制。方法：采用盲肠结扎穿孔术诱导的小鼠重度脓毒症模型。248只小鼠随机分为4组：假手术组、假手术＋氢气吸入组、重度脓毒症组和重度脓毒症＋氢气吸入组。氢气治疗分别为盲肠结扎穿孔术或假手术后1 h和6 h吸入2%氢气1 h。观察各组小鼠不同时间点生存率和血清HMGB1水平的变化。结果：重度脓毒症小鼠的7 d生存率为0%（P〈0.05）,且不同时间点血清HMBG1明显升高（P〈0.05）;氢气治疗可使重度脓毒症动物的7 d生存率升高至60%（P〈0.05）,且使血清HMBG1的水平明显降低（P〈0.05）。结论：氢气吸入能降低重度脓毒症小鼠血清的HMGB1水平并提高其生存率。

英文摘要：

Objective： To investigate the effect of hydrogen gas inhalation on survival rate and serum high mobility group box 1（HMGB1） levels in severe septic mice.Methods： Severe sepsis was induced by cecal ligation and puncture（CLP） operation in mice.A total of 248 mice were randomly divided into four groups：sham operation group（sham）,sham operation with hydrogen gas inhalation group（sham＋H2）,severe CLP group（severe CLP） and severe CLP with hydrogen gas inhalation group（severe CLP＋H2）.Hydrogen gas inhalation was given for 1 h at 1st and 6th h after CLP or sham operation,respectively.The survival rates and serum HMGB1 levels of all groups at different time points were measured.Results： The 7-d survival rates of severe CLP mice was 0%（vs Sham group,P0.05）,and the serum HMBG1 levels from h2 to h32 after CLP operation were significantly increased in severe CLP mice（vs Sham group,P0.05）.Hydrogen gas treatment increased the 7-d survival rate of severe CLP mice to 60%（vs severe sepsis group,P0.05） and significantly reduced the serum HMGB1 levels at different time points（vs severe sepsis group,P0.05）.Conclusion： Hydrogen gas inhalation can decrease the serum HMGB1 levels and increase the survival rate of rats with severe sepsis.