中文摘要：

目的：比较豫医卷毛大鼠与其他物种间表皮生长因子受体（EGFR）基因及其编码蛋白的序列差异。方法：采用RT-PCR方法对豫医卷毛大鼠EGFRcDNA序列进行克隆、测序,并借助生物信息技术对BN大鼠、家鼠、黄牛、猕猴和人EGFR基因的核苷酸序列及其编码蛋白的氨基酸序列进行同源性比较和系统遗传学分析。结果：获得了豫医卷毛大鼠EGFR基因全长4127bp的cDNA序列（GenBank登录号：HM801041）。豫医卷毛大鼠与大鼠、小鼠、牛、猴和人等5个物种EGFR基因编码区的核苷酸序列同源性分别为99.5%、93.1%、81.6%、83.3%和83.6%,其编码蛋白的氨基酸序列同源性分别是99.8%、96.1%、87.4%、89.2%和90.9%。结论：EGFR基因在进化过程中是一个高度保守并具有重要功能的＂持家基因＂。

英文摘要：

Aim：To study the difference of epidermal growth factor receptor（EGFR） gene and its coding protein between Yuyi curly hair rats and others species.Methods：RT-PCR technique was developed to clone and analyze the sequence of cDNA in EGFR gene from Yuyi curly hair rats,and biologic character of amino acid sequence encoding EGFR gene in different species （Norway rats,house mice,cattle,rhesus monkeys,and human）.Results：A full-length cDNA of Yuyi curly hair rats was 4 127 bp（Accession Number：HM801041）.The identities of nucleotide of EGFR gene were 99.5%,93.1%,81.6%,83.3%,and 83.6% by homologous comparison among Yuyi curly hair rats and Norway rats,house mice,cattle,rhesus monkeys,and human,and those of amino acid sequences were 99.8%,96.1%,87.4%,89.2%,and 90.9%,respectively.Conclusion：EGFR gene is a highly-conserved gene and important housekeeping gene.