中文摘要：

目的：对豫医卷毛大鼠的Krt71基因进行比较生物学分析。方法：采用PCR方法对豫医卷毛大鼠Krt71的cDNA序列进行克隆、测序，并与BN大鼠、小家鼠、家牛、家绵羊、家猫、苏门答腊猩猩和人的Krt71 cDNA序列及其编码产物的氨基酸序列进行同源性比较和系统遗传学分析。结果：获得了豫医卷毛大鼠Krt71基因全长1573 bp的cDNA序列（ GenBank登录号：KF303588）。豫医卷毛大鼠与BN大鼠、小家鼠、家牛、家绵羊、家猫、苏门答腊猩猩和人的同源性分别为99．9％、95．2％、88．5％、87．5％、88．0％、88．1％、88．1％，氨基酸序列的同源性分别为99．9％、98．9％、92．4％、91．2％、91．4％、92．3％、92．1％。结论：Krt71基因在哺乳动物进化过程中具有较高的保守性，是一个具有重要功能的“持家基因”。

英文摘要：

Aim：To study the difference of Keratin 71（Krt71） gene and its coding between Yuyi curly hair rats and other species .Methods：PCR technique was developed to clone the sequence of cDNA of Krt 71 gene from Yuyi curly hair rats, and biologic characters of nucleotide and amino acid sequence encoding Krt 71 gene in different species （ rattus norve-gicus, mus musculus, bos taurus, ovis aries, felis catus, pongo abelii and homo sapiens ） were analyzed.Results：A full-length cDNA of Yuyi curly hair rats was 1 573 bp（ Accession Number：KF303588 ） .The Krt71 gene nucleotide homology of Yuyi curly hair rats to rattus norvegicus , mus musculus, bos taurus, ovis aries, felis catus, pongo abelii,and homo sapiens was 99.9%,95.2%,88.5%,87.5%,88.0%,88.1% and 88.1%,and those of amino acid sequences was 99.9%, 98.9%,92.4%,91.2%,91.4%,92.3%and 92.1%,respectively.Conclusion：A high degree of conservation of Krt 71 gene exists among different mammalian taxons , and Krt71 gene is an important housekeeping gene .