中文摘要：

采用表面等离子体共振（SPR）方法，用鸡蛋黄抗体（IgY）取代传统免疫检测中哺乳动物抗体IgG作为识别分子偶联于CM5传感芯片上，对人血清中的转铁蛋白进行了检测．考察了Igy在传感芯片上的偶联条件及芯片的再生条件．结果表明，在pH=4．0，IgV浓度为100μg／mL，流速为5μL／min的最佳偶联条件下，SPR响应信号和转铁蛋白浓度在50～500ng／mL范围内具有良好的线性关系，检出限为39．56ng／mL，对人血清样品检测的日间变异系数〈8％，日内变异系数〈5％，平均回收率为86．22％～94．51％．

英文摘要：

Chicken egg yolk antibodies （IgY） instead of traditional mammalian antibodies were immobilized to a sensor chip CM5 for the detection of transferrin in human serum by surface plasmon resonance （SPR）. The conditions for immobilizing chicken anti-human transferring antibodies and regeneration were studied. Chicken egg yolk antibodies were immobilized under the condition of a flow rate of 5 μL/min, pH = 4. 0 and the concentration of 100μg/mL. The good linear range was obtained over 50-500 ng/mL, the detection limit was 39. 56 ng/mL. The determination of transferrin in human serum showed good reproduction and precision, intra-day CV was below 5%, and inter-day CV was below 8%, average recoveries ranged from 86. 22% to 94. 51%.