中文摘要：

目的：探讨担载紫杉醇纤维毡对卵巢癌细胞SKOV3的缓释效应及作用的有效性。方法：以聚左旋乳酸-聚乙二醇750（PLLA-PEG750）作为载体材料，采用电纺丝技术制备成载药率为2．50紫杉醇纤维毡，以高效液相色谱法检测紫杉醇纤维毡药物释放情况。将其分为实验组（2．5%紫杉醇纤维毡组）、纯药组（紫杉醇作用于SKOV3组）及对照组（不加药同期培养的SKOV3组）3个组，采用MTT法筛选并计算紫杉醇纤维毡对卵巢癌SKOV3的抑瘤率，采用流式细胞术对实验组进行细胞凋亡及细胞周期分析。结果：药物释放基本符合零级动力学；担载紫杉醇纤维毡体外抑瘤率达94．59％±1．160，接近或达到纯药组；流式细胞术表明，实验组作用于SKOV3 48h后，G2／M期细胞百分比明显增加，48h实验组凋亡率为20．02％±0．96%，而对照组凋亡率为8．09％±0．54%，两者比较差异具有显著性（P〈0．01）。结论：担载紫杉醇纤维毡具有明显缓释效应，体外作用于卵巢癌细胞SKOV3能够有效抑制肿瘤细胞增殖，阻止细胞周期进程。

英文摘要：

Objective To investigate the delayed release effect and the availability of the paclitaxel-loaded PLLA- PEG750 ultrafine fibers on ovarian cancer SKOV3 cell line. Methods Taking PLLA-PEG750 as the carrier, paclitaxel was well incorporated and dispersed uniformly in biodegradable PEG-PLLA750 fibers by using electrospinning method. The release of the drug was determined by high performance liquid chromatography. They were divided into three groups： experimental group （2.5% paclitaxel-loaded ultrafine fibers group）, pure drug group （paclitaxel on SKOV3 group）, control group （SKOV3 in the same culture period group）. Using MTT assay, the inhibitory rate of experimental group to the SKOV3 was sieved out and calculated. The apoptosis of SKOV3 cells was analyzed by flow cytometry. Results The release of the drug corresponded zero order kinetics. The inhibitory rate of the paclitaxel-loaded ultrafine fibers to SKOV3 in vitro was 94.59% ±1.16%, and approached or achieved the level in pure drug group. According to the result of flow cytometry, the percentage of G2/M of SKOV3 in experimental group 48 h after treatment was significantly up-regulated, the apoptotic rate inexperimental group （20.02 ± 0.96 %） was significantly higher than that in control group （8.09 ±0.54%） （P〈 0.01）. Conclusion The paclitaxel-loaded ultrafine fibers have conspicuous delayed release effect. It can inhibit the growth of SKOV3 cells and block the process of G2/M period in vitro.