中文摘要：

采用PC机为硬件平台，基于Linux操作系统，以Perl语言作为主要的程序编辑语言，整合SeqClean、TGICL、RepeatMasker、Blast、Bioperl等软件或模块，构建自动化、高通量的转录组分析系统。整个分析系统以三个自编的Perl脚本为主程序，其中以assemble．pl为主程序的序列组装，以annotFun．pl为主程序的功能注释和以annot—GO．pl为主程序的功能分类。通过分析粤油20在叶斑病诱导下获得的8328个叶片EST，进一步验证该系统的稳定性和可靠性。本文构建的花生转录组分析系统通过三个主程序能够自动、简洁、高通量地完成花生转录组数据的组装、注释与分类，为花生功能基因组学研究提供有价值的生物信息，也为其他生物信息平台的构建提供借鉴。

英文摘要：

A high -throughput analysis system for peanut transcriptome was constructed based on a PC ma- chine, Linux operating system and free software such as SeqClean,TGICL,RepeatMasker,Blast and Bioperl. The a- nalysis system included three sub - systems with three self - developed Perl scripts. The three sub - systems were the sequence assembled with a Perl script assemble, pl, the functional annotation with an annotFunc, pl and the GO classification with an annot GO. pl. To validate the analysis system, we annotated a total of 8 328 EST sequences from a cDNA library constructed with leaf spot infected leaves of Yueyou 20. The results indicated that the system, combining publicly free software with the scripts written with Perl language, made peanut transcriptome data analysis easy and automatic. This analysis system will play a role in peanut functional genomics and contribute to the construction of analysis platforms for other researchers.