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Hydrolysis Reaction of N-Phosphoryl-α-, β- and γ-amino Acids Studied by HPLC
  • ISSN号:1001-604X
  • 期刊名称:《中国化学:英文版》
  • 时间:0
  • 分类:O629.7[理学—有机化学;理学—化学] O657.7[理学—分析化学;理学—化学]
  • 作者机构:[1]The Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou, Henan 450052, China, [2]The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology of the Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China
  • 相关基金:Project supported by the National Natural Science Foundation of China (Nos. 20572061, 20672104).
中文摘要:

N-的水解作用反应( O , O-diisopropyl ) phosphoryl-L --丙氨酸( DIPP-L --翼),N-( O , O-diisopropyl )- phosphoryl-D --丙氨酸( DIPP-D --翼),N-( O , O-diisopropyl ) phosphoryl --丙氨酸( DIPP --翼)并且N-( O ,O- diisopropyl ) phosphoryl --氨基的奶油的酸( DIPP --骆驼毛的织物),被 HPLC 和他们的水解作用反应学习运动方程被获得。在酸条件下面, DIPP-L 的反应率 -- 翼接近了 DIPP-D 的 -- 翼和一样的规则在 DIPP 之间是真的 -- 翼和 DIPP -- 骆驼毛的织物。同时, DIPP-L/D 的反应率 -- 翼作为 DIPP 的作为 10 次 -- 翼或 DIPP -- 骆驼毛的织物。在基本条件下面, DIPP 的水解作用反应 -- 翼和 DIPP -- 骆驼毛的织物几乎没发生并且 DIPP-L/D 的反应率 -- 翼在酸条件下面关于那的 1/10。而且,理论计算进一步从精力的看法照亮了水解作用率的差别。结果将提供一些有用线索自然为什么选择了氨基酸然而并非其它种类似物为蛋白质脊梁。

英文摘要:

The hydrolysis reactions of N-(O,O'diisopropyl)phosphoryl-L-α-alanine (DIPP-L-α-Ala), N-(O,O'diisopropyl)- phosphoryl-D-α-alanine (DIPP-D-α-Ala), N-(O,O'-diisopropyl)phosphoryl-β-alanine (DIPP-β-Ala) and N-(O,O'-diisopropyl)phosphoryl-γ-amino butyric acid (DIPP-γ-Aba), were studied by HPLC and their hydrolysis reaction kinetic equations were obtained. Under acid conditions, the reaction rate of DIPP-L-α-Ala was close to that of DIPP-D-α-Ala and the same rule was true between DIPP-β-Ala and DIPP-γ-Aba. Meantime, the reaction rate of DIPP-L/D-α-Ala was as 10 times as that of DIPP-β-Ala or DIPP-γ-Aba. Under basic conditions, the hydrolysis reactions of DIPP-β-Ala and DIPP-γ-Aba almost did not take place and the reaction rate of DIPP-L/D-α-Ala was about 1/10 of that under acid conditions. Moreover, theoretical calculation further illuminated the differences of the hydrolysis rate from the view of energy. The results would provide some helpful clues to why nature chose a-amino acids but not other kinds of analogs as protein backbones.

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期刊信息
  • 《中国化学:英文版》
  • 主管单位:
  • 主办单位:中国化学会
  • 主编:
  • 地址:上海市枫林路354号中科院上海有机化学研究所
  • 邮编:200032
  • 邮箱:
  • 电话:021-54925243
  • 国际标准刊号:ISSN:1001-604X
  • 国内统一刊号:ISSN:31-1547/O6
  • 邮发代号:4-646
  • 获奖情况:
  • 中国期刊方阵“双高”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),荷兰文摘与引文数据库,美国科学引文索引(扩展库),日本日本科学技术振兴机构数据库,英国英国皇家化学学会文摘
  • 被引量:175