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中文摘要：
对两种杂交石斑鱼子一代（青龙斑和虎龙斑）及其亲本（斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼）的线粒体DNA细胞色素氧化酶Ⅰ（COⅠ）基因序列进行了测序分析。在15个样本中，同源序列片段（1551bp）中共检测到9个单倍型和249个核苷酸多态位点。序列差异分析和遗传距离比较结果显示，核苷酸序列同源性在88．1％-100％之间，无明显遗传分化。虎龙斑的2个COⅠ基因单倍型与母本棕点石斑鱼单倍型的同源性为99％和100％，而与父本鞍带石斑鱼的同源性均为88．1％。青龙斑的2个单倍型与母本斜带石斑鱼的3个单倍型的同源性在99．7％-100％之间，而与父本鞍带石斑鱼的同源性分别为89．3％和89．4％，结果表明两种杂交子一代在线粒体DNACOⅠ基因上严格遵循母性遗传规律。
英文摘要：
In order to identify the phylogenetic relationship among two grouper hybrids （ Qinglong grouper and Hulong grouper） and their parents （ Epinephelus coioides 9, Epinephelus coioides ♀ and E. lanceolatus ♂ ）, their mitochondrial cytochrome oxidase subunit I （CO I ） gene fragments were amplified by PCR, and the 1551 base-pair nucleotide sequences of CO I were examined. The results show that all 15 sequences are grouped into 9 haplotypes and 249 nucleotide polymorphic loci in CO I gene fragments. Sequence divergences and genetic distance analysis showed all the groupers shared 88.1% to 100% similarities in nucleotide sequence, indicating there is no significant genetic difference among these groupers. The two CO I haplotypes of Hulong grouper showed 99% and 100% similarities with the haplotype of the female parent E. fuscoguttatus, respectively, but only 88.1% similarity with the male parent E. lanceolatus. The two CO I haplotypes in Qinglong grouper had similarities of 99.7% to 100% with the three haplotypes of the female parent E. coioides, and only of 89.3% and 89.4% with the male parent E. lanceolatus. The results indicated that the two grouper hybrids were inherited from their maternal parent in mitochondrial CO I genes.
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Isolation and Characterization of Microsatellite Markers from 1 the Brown-marbled Grouper, Epinephel
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