中文摘要：

目的：测定川芎药材及芎冰鼻腔喷雾剂的指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法：高效液相色谱法,Phenomenex Hyperclone BDS C18（4.00 mm×250 mm,5μm）,甲醇1%冰乙酸为流动相,梯度洗脱,322 nm检测。结果：川芎药材和芎冰鼻腔喷雾剂指纹图谱峰数多,分离好,有相关性,精密度,重复性,稳定性均〈3%。结论：本方法科学、准确、专属性好,可为芎冰鼻腔喷雾剂及含川芎的中成药控制质量提供可靠的科学依据。

英文摘要：

Objective：To establish sensitive and specific HPLC fingerprint for controlling the quality of Rhizoma Chuanxiong and Xiongbing Nasal spray.Method： HPLC method was applied for quality assessment of Xiongbing Nasal spray.HPLC analysis was performed on a Phenomenex Hyperclone BDS C18（4.00 mm × 250 mm,5 μm） by gradient elution using methanol-1% acetic acid solution as mobile phase.Flow-rate was 1 mL.min-1.The detection wavelength was at 322 nm.Result： Rhizoma Chuanxiong and Xiongbing Nasal spray were closely related to each other.Conclusion： The method is rapid and accurate.It can be used for determination of Xiongbing Nasal spray and Chinese formulated medicine with it.