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Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920

ISSN号：1004-9541
期刊名称：《中国化学工程学报：英文版》
时间：0
分类：TS205.7[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程] TS201.3[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程]
作者机构：[1]Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China, [2]Shaanxi R&D; Centerof Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China, [3]Shaanxi R&D; Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China
相关基金：Supported by the National High Technology Research and Development Program of China （2007AA03Z456）, the National Natural Science Foundation of China （20776119, 21076169 and 31000019）, the Xi＇an Research and Development Program （NC08005, YF07078）, the Scientific Research Program of Shaanxi Provincial Department of Education, China （08JK452, 08JK453, JG08181, 2010JC21, 2010JS107, 2010JS108, 2010JK876 and 2010JS109）, Shaanxi Provincial Scientific Technology Research and Development Program （2007K06-03, 2010JQ2012, SJ08B03）, the Specialized Research Fund for the Doctoral Program of Higher Education of China （20096101120023, 20096101110014）, NWU Graduate Innovation and Creativity Funds （08YSY17）, and Shaanxi Key Subject Program, China.

关键词：冷冻干燥过程, 长双歧杆菌, 科学研究所, 变量优化, 化学成分, 保护剂, 农业部, BOX-BEHNKEN设计, Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology

中文摘要：

结冰弄干在微生物的生存能力上有有害效果。在这个困难前面，现在的学习采用反应表面方法论优化保护的代理人的化学作文在 freeze-drying 期间寻找 Bifidobacterium longum BIOMA 5920 的最大的生存能力。通过单个杀虫撒布剂的比较分析，复杂保护的代理人在 Bifidobacterium 生存能力上显示出更好的效果。似人类的骨胶原(HLC ) ， trehalose 和甘油被 Box-Behnken 设计作为重要因素证实。为这三个变量的优化公式如下被测试：HLC 1.23% ， trehalose 11.50% 和甘油 4.65% 。在这个公式下面，生存能力是 88.23% ， 39.67% 与控制相比更高。可行计数是 1.07 ? 吠条 ' 讟 E??????????????? 楂汯慥档湩 ?? à

英文摘要：

Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the compara- tive analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen （HLC）, trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows： HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10 9 cfu·g-1 , greatly exceeding the minimum viable count requirement （10 6 cfu·g-1 ）.

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