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GF-β1对诱导肾间质成纤维细胞活化并表达MMP-9及TIMP-1的影响

ISSN号：2095-5227
期刊名称：《解放军医学院学报》
时间：0
分类：R34[医药卫生—基础医学]
作者机构：[1]解放军总医院肾病中心暨国家重点实验室,北京100853
相关基金：国家自然科学基金项目（30130220;30873345）

关键词：转化生长因子-β1, 肾间质成纤维细胞, 表型转化α-平滑肌肌动蛋白, 纤维连接蛋白, 基质金属蛋白酶9, 组织金属蛋白酶抑制剂1, TGF-β1, renal interstitional fibroblast, phenotype-differentiation α-smooth muscle actin, fibronectin, MMP-9, TIMP-1

中文摘要：

目的研究转化生长因子-β 1 （transforming growth factor beta-1 ，TGF-β 1 ）诱导的肾间质成纤维细胞（NRK-49F）发生表型转化时基质金属降解酶MMP-9 及其抑制因子TIMP-1 表达的变化及对分泌纤维连接蛋白（fibronectin，FN）的影响。方法以不同浓度hTGF-β 1 （0 ng/ml 、0.5 ng/ml、1 ng/ml、2 ng/ml、5 ng/ml、10 ng/ml）刺激NRK-49F 细胞48 h，分别应用ELISA法检测细胞上清FN 的浓度，免疫荧光检测细胞表型标记物α-SMA、Vimentin 的表达，Western blot、Northern blotting 方法检测MMP-9、TIMP-1 的基因及蛋白质表达。结果 0.5 ～ 10 ng/ml TGF- β 1 随刺激浓度增高NRK-49F 细胞表达α-SMA明显增多、Vimentin 明显减少，细胞上清中FN 的含量显著增加（P ＜ 0.05 或P ＜ 0.01）；同时，TGF-β 1 浓度＞ 2 ng/ml 时显著上调TIMP-1 mRNA 及蛋白表达水平（P ＜ 0.05 或P ＜ 0.01）；以上效应均呈浓度依赖性，但对细胞MMP-9 mRNA 及蛋白表达无明显影响（P ＞ 0.05）。结论 TGF- β 1 能诱导肾间质成纤维细胞活化，使FN 的分泌增多，该作用可能与TGF-β 1 上调了TIMP-1 基因及蛋白质水平，进而减少细胞外基质（extracellular matrix，ECM）的降解有关。

英文摘要：

Objective To study the changes on MMP-9 and TMP-1 expression when renal interstitial fibroblast cells （NRK-49F） occurred phenotypic transform induced by transforming growth factor beta-1 （TGF- β 1 ）, and its effects on the secretion of fibronectin （FN）. Methods Renal interstitial fibroblast cells （NRK-49F） of rats were stimulated by hTGF- β 1 at different concentrations of 0 ng/ml, 0.5 ng/ml, 1 ng/ml, 2 ng/ml, 5ng/ml, 10 ng/ml. Immune fluorescence was used to measure the expression of cell phenotype sign α -SMA and Vimentin, and ELISA was used to detect the concentration of FN in supernatant, while Western Blot and Northern Blotting technique was used to measure the expression of MMP-9 and TIMP-1. Results TGF- β 1 at the concentration of 0.5 ng/ml - 10 ng/ml induced the expression of α -SMA which represented myofibroblast appearance, with increasing concentration of TGF- β 1 , the expression of α -SMA gradually increased and Vimentin decreased significantly, furthermore FN secretion were driven up greatly （P 〈 0.05 or P 〈 0.01）, while TGF- β 1 at the concentration of 2 ng/ml markly drove up the expression of TIMP-1 mRNA and protein（P 〈 0.05 or P 〈 0.01） , those effects showed concentration-dependent form. However, TGF- β 1 at above concentration had no remarkable effect on MMP-9 mRNA and protein expression （P 〉 0.05）. Conclusion TGF- β 1 can induce renal interstitial fibroblast activation to be myofibroblast, and promote fibronectin secretion, whose effects may be related to up-regulation of TIMP-1 expression so as to restrain ECM decompounded.

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