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三七总皂苷防治大鼠腺嘌呤肾间质纤维化作用的实验研究

ISSN号：1674-3849
期刊名称：《世界科学技术：中医药现代化》
时间：0
分类：R285.5[医药卫生—中药学;医药卫生—中医学] R692[医药卫生—泌尿科学;医药卫生—临床医学;医药卫生—外科学]
作者机构：[1]北京中医药大学,北京100029
相关基金：教育部“长江学者和创新团队发展计划”资助项目（IRT0413）：中医药千预多器官纤维化与异病同治、同病异治科学内涵的研究,负责人：牛建昭;国家自然科学基金重点资助项目（30130220）：复方鳖甲软肝方防治多脏器纤维化的作用机理研究,负责人：牛建昭;高等学校学科创新引智计划资助（B07007）：中西医结合学科创新引智基地,负责人：牛建昭.

关键词：三七总皂苷, 肾间质纤维化, 腺嘌呤, 大鼠, panax notoginseng saponins, Tubulointerstilial Fibrosis, adenine, rat

中文摘要：

目的：观察三七总皂苷对大鼠腺嘌呤性肾间质纤维化的防治作用。方法：将Wister大鼠58只，随机分为5组，正常组、假手术组、模型组、尿毒清组和三七总皂苷组。模型组、尿毒清组和三七总皂苷组大鼠，手术切除左侧肾脏，而假手术组手术取出左侧肾脏，剥离深包膜但不切除肾脏。模型组、尿毒清组、三七总皂苷组以腺嘌呤180mg／kg灌胃，并同时给予尿毒清和三七总皂苷治疗，实验在第2周和第4周时称重，并留取24h尿液。第4周末处死大鼠，留尿、取血测定尿、血Ca^2＋、P^3-及Scr、BUN，取肾组织称重、福尔马林固定，并进行肾组织染色。用SPSS11．0进行统计学处理。结果：三七总皂苷组与模型组比较，可以明显改善肾间质纤维化大鼠的血Ca^2＋、P^3-（P〈0．05），提高血RBC、Hb水平，降低血清Scr、BUN（P〈0．05），减少肾组织中腺嘌呤结晶的沉积，减轻肾脏病理损害。结论：三七总皂苷通过减少腺嘌呤性大鼠肾组织中腺嘌呤结晶的沉积、降低P^3-，提高Ca^2＋,RBC、Hb水平，改善肾功能，从而对腺嘌呤性大鼠肾间质纤维化有明显的防治作用。

英文摘要：

We investigated the therapeutic effects of panax notoginseng saponins （PNS） on Tubulointerstilial Fibrosis in an adenine nephropathy rat model. The study had employed 58 Wister rats, which were randomly divided into 5 groups： normal group, sham operation group, model group, Niaoduqing group, and PNS group. Rats in the model group, Niaoduqing group, and PNS group had their left kidney taken away through germ free operation. Rats in the sham operation group were peeled off the kidney envelope without renal excision. One week after the operation, rats in the model group, Niaoduqing group, and PNS group were intragastrically administrated with Adenine （180mg/kg/d）. Meanwhile, the Niaoduqing group were gastrically perfused with Niaoduqing, and PNS group intraperitoneally injected with PNS. Body mass were weighed using an electronic scale, and 24-hour urine samples were collected for urine protein detection; at an interval of 2 and 4 weeks. At the end of the fourth week, all the rats were sacrificed, with their blood samples being tested for Ca^2＋,P^3-,Scr and BUN. Kidneys were weighed, fixed by formalin, and examined pathohistologically. The collected data were statistically analyzed using SPSS 11.0 package. Results showed that serum Ca^2＋, P^3 , RBC, and Hb were obviously improved, and serum Scr and BUN were reduced in the PNS group. Compare with the model group, there was a statistic significance （P〈0.05）. Pathohistological examination also showed that PNS could lessen the deposition of crystallized adenine, and abate the histomorphological injury of kidney. We believe that PNS can improve the renal function by lessening the deposition of crystallized adenine, and elevate the level of Ca^2＋, RBC, and Hb, while reducing the level of P^3- in the blood. PNS, therefore, has a therapeutic effect on tubulointerstilial fibrosis in an adenine nephropathy rat model.

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