中文摘要：

目的：评价戴氏绿僵菌的抗微生物潜力,并明确其有效部位。方法：采用琼脂扩散法测定戴氏绿僵菌各分离部位的抗病原细菌作用,利用生长速率法测定其抗病原真菌活性,并采用微量稀释法和平板菌落计数测定有强抗菌活性部位的最低抑菌浓度（MIC）及最低杀菌浓度（MBC）。结果：戴氏绿僵菌能显著抑制革兰氏阳性和阴性临床病原细菌以及植物病原真菌的生长,抑菌谱较广。戴氏绿僵菌抗微生物活性的有效成分主要存在其发酵液乙酸乙酯以及菌丝体乙酸乙酯和氯仿等3个萃取部位,不同的萃取部位抗菌谱及抗菌能力有较大的差异。结论：戴氏绿僵菌具有强的广谱抗微生物活性,是一种值得关注的食药用生物资源。

英文摘要：

Objective：To investigate the antimicrobial potential of Metarhizium taii and its corresponding active fractions.Methods：The agar diffusion method and growth-rate assay were employed individually to measure the antibacterial and antifungal activities of M.taii.The microdilution and disk colony count was used to test the minimal inhibitory concentration and minimal bacterial concentration.Results：The extraction fractions of M.taii could evidently inhibi the growth of Gram positive bacteria such as Staphylococcus aureus,Bacillus subtilis,Gram negative bacteria such as Escherichia coli,Pseudomonas aerμginosa,and Klebsiella pneumoniae,and Enterococcus faecalis,and phytopathogenic fungi such as Rhizoctonia solani,Fusarium oxysporum,hereby M.taii had a broad spectrum antimicrobial effect including potent antibacterial and antifungal activities.The antimicrobial active ingredients of M.taii mainly existed in three extraction fractions including ethyl acetate extract in fermented broth,chloroform,and ethyl acetate extracts in mycelia.However there was an evident discrepancy on the antibacterial spectrum and competence for different active extraction fractions.Conclusion：M.taii possess potent and broad-spectrum antimicrobial potential,and it is a good edible and medicinal resource for the functional food development and the discovery of new antimicrobial drug.