中文摘要：

目的以耐尔蓝A硫酸盐为探针，建立一种共振散射（Rs）技术测定痕量肝素钠的新方法。方法在pH 3．29的Britton-Robinson缓冲溶液中，肝素钠与耐尔蓝A硫酸盐反应，导致体系的共振散射强度增强，最强共振散射峰为732nm。本文研究了耐尔蓝A硫酸盐-肝素钠体系的光谱特征、适宜的反应条件和共存物质的影响。结果肝素钠的浓度在0．16～4．52μg／mL范围内与732nm处的共振散射强度增强呈良好的线性关系，其回归方程为△IRS=3．22＋50．48c（R：0．9991），检出限（3σ）为3．33ng／mL，该方法已用于肝素钠注射液的分析，回收率在95．6％-104．2％之间。结论建立了一种简便、快速、灵敏的RS法测定痕量肝素钠的方法。

英文摘要：

Objective Using nile blue A sulphate as a spectrum probe, a new method for the determination of trace heparin sodium by resonance light scattering （RS） has been developed.Methods In pH 3.29 Britton-Robinson solution, heparin sodium can react with nile blue A sulphate to form associated particles , which enhanced RS signals greatly and produced new RS spectrum. The strongest RS peak is at 732nm. In this paper, the spectral characteristic of nile blue A sulphate - heparin sodium system,the optimum reactive conditions and the effect of co-existing substances were investigated in details. Results The RS intensity is directly proportional to the concentration of heparin sodium in the range of 0.16-4.52μg/mL at 732nm.Its regress equation is △IRS=3.22＋50.48c（R= 0.9991）, with the detection limit 3.33ng/mL（3σ）. The method is applied to the analysis of heparin sodium in injection sample,and the recovery is in the range of 95.6%-104.2%. Conclusions A simplicity, rapidity and high sensitive RS method is developed for the determination of trace heparin sodium.