中文摘要：

为提高酶促合成食品添加剂乙酸肉桂酯的效果，选用荧光假单胞脂肪酶（PFL）为生物催化剂，肉桂醇为底物，乙酸乙烯酯为酰化试剂和溶剂，研究了PFL在反应器内壁上的固定化、及其催化转酯反应合成乙酸肉桂酯的动力学。结果表明，相对于塑料（PMMA和PET），在玻璃壁上的固定化PFL，吸附牢固，活性高。酶的固定化中，水可以明显优化PFL，但是不能稳定PFL；如果添加亲水性大分子羧甲基纤维素（CMC），则可以更好地稳定PFL。催化转酯18h，在玻璃壁上的CMC-固定化PFL可转化底物99％，再次使用时仍可转化83％的底物；而水-固定化PFL可转化底物98％，但再次使用时仅转化底物76％；酶粉转化底物86％，再次使用时转化底物62％。可见，在玻璃载体上的CMC-固定化PFL可更有效地催化合成乙酸肉桂酯。

英文摘要：

For enhancing the enzyme-catalyzed effect in synthesis of cinnamyl acetate, Pseudomonas fluorescens Iipase （PFL） was employed as biocatalyst used in reaction system constituted by cinnamyl alcohol as substrate and vinyl acetate as acylation reagent and solvent, to study the immobilization of PFL on the inner wall of reactors and the enzyme kinetics in synthesis of cinnamyl acetate via catalyzing transesterification of cinnamyl alcohol with vinyl acetate. The results showed that PFL adsorbed firmly on glass wall rather than on plastic （PMMA and PET） wall and also the former behaved an activity obviously higher than the latter. Water could nicely optimize but ineptly stabilize PFL unless hydrophilic macromolecular carboxymethyl cellulose （CMC） imported in. After 18 h, on glass wall CMC-immobilized PFL and its recycling could transform 99% and 83% of substrate severally, comparatively which were 98% and 76 % when water-immobilized PFL were employed for the first and second. And native PFL powder and its recycling only transformed 86% and 76% of substrate, respectively. It was obvious that on glass wall CMC- immobilized PFL could more effectively catalyze transesterification for synthesis of cinnamyl acetate.