中文摘要：

通过试验研究不同水平纳米锌对半滑舌鳎消化酶的影响。试验鱼选取体重为（52.68±0.13）g,随机分成7组,每组3个重复,分别投喂添加量为0、30、60、90、120和180 mg/kg的纳米锌,另外一组添加60 mg/kg的无机锌（Zn SO4·7H2O）,经过84 d喂食后每个重复随机选取6尾试验鱼进行指标测定。结果表明：①肝胰脏及前肠蛋白酶活力分别在纳米锌添加量为90、60 mg/kg时达到最大值,且90 mg/kg时肝胰脏蛋白酶活力显著高于其他各组（P〈0.05）;纳米锌添加量为90 mg/kg及无机锌组对中肠蛋白酶的影响均显著高于除60 mg/kg组之外其它各组（P〈0.05）;60 mg/kg组对后肠蛋白酶的影响显著高于对照组及30 mg/kg组（P〈0.05）,对其他各组无显著性影响（P〉0.05）。②肝胰脏及肠道的脂肪酶活力最大值均出现在纳米锌添加量为0 mg/kg组,且中肠脂肪酶活力显著高于其他各组（P〈0.05）。③当纳米锌添加量为120 mg/kg时肝胰脏及前肠的淀粉酶活力均呈最大值,且肝胰脏淀粉酶活力显著高于除90 mg/kg组之外其他各组（P〈0.05）,前肠淀粉酶活力显著高于除180 mg/kg组之外其他各组（P〈0.05）;中肠淀粉酶活力在无机锌组呈现最大值且显著高于其他各组（P〈0.05）;对后肠淀粉酶的影响也在无机锌组呈现最大值,但除显著高于90 mg/kg组外（P〈0.05）,对其他各组无显著性影响（P〉0.05）。

英文摘要：

This test studied effect of different levels of nano-zinc on Cynoglossus semilaevis digestive enzymes. The initial weight of C. semilaevis is（52.68±0.13） g were randomly distributed into 7 groups of each 3 replicates 35 individuals in each replicate in this experiment. Experimental diets were supplemented with six levels of nano-zine（0, 30, 60, 90, 120, 180 mg/kg）, and another group is supplemented with inorganic zine（ZnSO4·7H2O） of 60 mg/kg. After 84-d feeding, the parameters were measured. Results indicated that ① The protease activity of hepatopancreas and foregut reached maximum value in 90 mg/kg and 60 mg/kg, and the protease activity of hepatopancreas was significantly higher than other groups in 90 mg/kg（P〈0.05）; the protease activity of midgut were significantly higher than those in other groups except 60 mg/kg in the group of 90 mg/kg and inorganic zinc（P〈0.05）; in 60 mg/kg, the protease activity of hindgut were significantly higher than contrast group and 30 mg/kg. ② The maximum value of lipase activity in hepatopancreas and intestine were found in 0 mg/kg group, and the midgut lipase activity was significantly higher than the other groups（P〈 0.05）. ③ When the addition of nano-zinc was 120 mg/kg, the anaylase activity in hepatopancreas and foregut showed a maximum value, and amylase activity of hepatopanereas was significantly higher than other groups except the 90 mg/kg（P〈0.05） and amylase activity of foregut was significantly higher than other groups except 180 mg/kg（P〈0.05）; the amylase activity of midgut showed a maximum value, and significantly higher than other groups in the group of inorganic zinc （P〈0.05）. Effect of amylase activity of hindgut also showed maximum value in the group of inorganic zinc, and significantly higher than the group of 90 mg/kg （P〈0.05）, without significant influence for the other groups.