为了解连翘、黄连、穿心莲对半滑舌鳎血液抗氧化能力的影响。本文采用单因子试验设计,将连翘、黄连、穿心莲按0、5、10、15 g/kg添加到饲料中,7,14,21 d后取样,检测半滑舌鳎血液总超氧化物歧化酶(T-SOD),过氧化氢酶(CAT),总抗氧化能力(T-AOC),丙二醛(MDA),总胆固醇(T-CHO),甘油三酯(TG)等指标。结果表明,连翘组:14 d后,5 g/kg组T-SOD活力达到最高。21 d后,5 g/kg组CAT活力最高。14 d后15 g/kg组MDA值显著低于对照组。黄连组:14 d后15 g/kg组T-SOD活力达到最高。21 d后,15 g/kg组T-AOC活力显著高于对照组。穿心莲组:14 d后,10 g/kg组T-SOD活力,CAT活力及T-AOC活力都达到最高。21 d后,5g/kg组MDA值显著低于对照组。10 g/kg组T-CHO值和TG值在7 d后均显著高于对照组。结果显示,连翘、黄连及穿心莲单独添加对半滑舌鳎血液抗氧化指标均有不同程度的影响。
The study was conducted to understand the effect of Forsythia suspensa, Coptidis rhizoma, Andrographis paniculata on the blood antioxidant function in Cynoglossus semilaevis. A factorial experiment was designed to evaluate the blood parameters (the total superoxide dismutase ( T-SOD ), total antioxidant capacity ( T-AOC ), catalase (CAT), malonaldehyde (MDA), triglyceride (TG), total cholesterol (T-CHO)) in C. semilaevis after 7 days, 14 days and 21 days. Fish were fed diets containing different doses ofE suspensa, C. rhizoma, A. paniculata (0, 5, 10, 15 g/kg) . The results, diet supplemented with 5 g/kg E suspensa resulted in significantly highest T-SOD activity after 14 days, diet supplemented with 5 g/kg of E suspensa resulted in significantly highest CAT activity 21 days, diet supplemented with 15 g/kg F. suspensa resulted in significantly lower MDA content than the control group after 14 days. Diet supplemented with 15 g/kg C. rhizoma resulted in significantly highest T-SOD activity after 14 days. Diet supplemented with 15 g/kg of C. rhizoma resulted in significantly higher T-AOC activity than the control group after 21 days. Diet supplemented with 10 g/kg A. paniculata resulted in significantly highest T-SOD activity, CAT activity and T-AOC activity after 14 days. Diet supplemented with 5 g/kg A. paniculata resulted in significantly lower MDA content than the control group after 21 days. Diet supplemented with 10 g/kg A. paniculata resulted in significantly higher T-CHO content and TG content than the control group after 7 days. The results indicated that E suspensa, C. rhizoma, A. paniculata added individually had different degrees of influences on blood antioxidant parameters in C. semilaevis.