中文摘要：

以川芎为试材,从叶片中提取总RNA,经过RT-PCR获得甜菜碱醛脱氢酶（Betaine aldehyde dehydrogenase）基因的cDNA,纯化后与pMD18-T载体连接,转化大肠杆菌Top10,获得甜菜碱醛脱氢酶全长基因序列,以期构建植物表达载体。结果表明：甜菜碱醛脱氢酶全长核苷酸长度为1 527bp,编码508个氨基酸。与GenBank中已发表序列HM35276进行比较,核苷酸同源性为100%。将该基因片段克隆到植物表达载体pBI121中,构建重组质粒pBI121/Betaine alde-hyde dehydrogenase,并将所获重组质粒经过双酶切和PCR处理后进行序列测定,证实表达载体上含有目的片段,且连接、构建正确,为BADH的进一步表达奠定了基础。

英文摘要：

Taking Liqusticurn chuanxiong as material, the total RNA was extracted from leaf of Liqusticum chuanxiong and cDNA of Betaine aldehyde dehydrogenase gene were obtained using RT-PCR. The purified RT-PCR products were constructed into pMD18-T vector. The results showed that the full length of BADH gene consists of 1 527 bp, which encoded 508 amino acids. The homology of its nucleotide sequence with HM35276 was 100%. Furthermore,the aim gene was cloned into plant expression vector pBI121 and a recombinant plasmid pBI121/Betaine aldehyde dehydrogenase were constructed successfully according to double enzyme digestion,PCR and sequencing.