中文摘要：

为提高花生中黄曲霉毒素B1的检测效率和准确性，试验通过对提取溶剂、色谱条件和质谱条件的优化，建立了免疫亲和柱-超高效液相色谱串联质谱（UPLC—MS／MS）快速测定花生中黄曲霉毒素B1的方法，并对方法进行了评估。结果表明，用甲醇-水（60：40，V／V）对样品进行提取，采用黄曲霉毒素免疫亲和柱萃取、净化，以0．1％甲酸水溶液、0．1％甲酸乙腈溶液作为超高效液相色谱的流动相，在电喷雾离子源（ESI）正离子模式下采用多反应监测（MRM）对花生中黄曲霉毒素B1进行定性、定量检测，在5．5min内完成一个样品的分析。结果表明，黄曲霉毒素B1在0．1-56．0ng／mL的线性定量范围，相关系数高达0．99942，检出限低至0．02μg／kg，定量限精确至0．1μg／kg，低、中、高浓度回收率为82%-92％，相对标准偏差〈5％。该方法具有前处理简单、净化效果好和准确度高的优点，适用于花生等复杂基质样品的黄曲霉毒素B1定性和定量检测。

英文摘要：

Immunoaffinity column purification and UPLC-MS/MS method were established to determine aflatoxin B1 in peanut for improving the detection efficiency and accuracy of aflatoxin B1 through the test of extraction solvent, chromatographic condition and mass spectrometer conditions. Aflatoxin B1 was extracted from peanut, then the extract was filtered and purified by immunoaffinity column before being injected into UPLC-MS/MS to determine aflatoxin B1. The samples were extracted with methanol-aqueous solution （60 ： 40, V/V）, cleaned up and enriched by immunoaffinity column. Aflatoxin B1 was successfully detected by UPLC-MS/MS under positive ion mode and the multiple reaction monitoring （MRM） with the mobile phaseof 0.1% formic acid in water and 0.1% formic acid in acetonitrile within 5.5 min. The results showed that calibration curve presented good linear relationship （correlation coefficients 0.999 42） when the mass concentration of aflatoxin B1 ranged from 0.1 ng/mL to 56.0 ng/mL; The limit of detection and the limit of quantification of the method were 0.02 μg/kg and 0.1 μg/kg, respectively. Sample recoveries at three spiking levels were in the range of 82% -92%, RSD〈 5%. Optimize the condition of UPLC and the conditions of pre-extracted method had the advantages of simplicity, rapidity, precision, good reproducibility and high recovery, so that it could be suitable for the identification and quantification of aflatoxin B1 in complex matrixes.