该文研究了含酶反胶束体系后萃花生蛋白的过程,通过考察体系 pH、KCl 浓度、温度和振荡时间等因素,对花生蛋白后萃率进行研究,并采用 JMP10.0软件进行定制实验设计,以花生蛋白的后萃率为响应值,建立数学模型,确定花生蛋白后萃过程的最佳工艺条件:缓冲液的 pH 为8.06, KCl 浓度为1.53 mol/L,温度为40℃,萃取时间为120 min,在此条件下进行试验获得最佳后萃率为(78.43±0.87)%。
This paper studied the backward extraction of peanut protein by enzyme-containing reverse micelles. And the effects of the pH of buffer solution,KCl concentration,the temperature of extraction and oscillation time were investigated. The study designed by custom design function of JMP software and establish the mathematical model of peanut protein extraction. The optimum conditions of backward extraction conditions of peanut protein in enzyme-containing reverse micelles were the pH 8.06,KCl concentration 1.53 mol/L,temperature 40 ℃ and oscillation time 120 min. And the backward extraction rate was(78.43±0.87)%.