中文摘要：

目的研究蛋白激酶C（PKC）对豚鼠近视眼视网膜Mtiller细胞中酪氨酸羟化酶（TH）、诱导型NO合成酶（iNOS）、神经型NO合成酶（nNOS）、碱性成纤维细胞生长因子（bFGF）、转化生长因子B（TGFl3）基因表达的调控作用。方法眼罩遮盖法建立豚鼠近视眼模型，酶消化法原代培养其视网膜Müller细胞，GFAP免疫组织化学染色进行细胞鉴定。根据干预因素不同，把Müler细胞分为正常对照组、近视组、近视＋GF109203X组、近视＋PMA组和近视＋DMSO组。RT—PCR检测TH、iNOS、nNOS、bFGF和TGFβmRNA的表达情况。结果与正常对照组比较，近视眼视网膜Mtiller细胞iNOS、nNOS、bFGF和TGFβmRNA表达上调，THmRNA表达下调（P〈0．05）。PKC激活剂（PMA）激活PKC后，近视眼视网膜Müller细胞表达nNOS、iNOS、TH和bFGFmRNA上调（P〈0．05）；GF109203X抑制PKC活性后，这些因子的mRNA表达下调（P〈n05）。结论豚鼠近视眼视网膜Müller细胞nNOS、iNOS、bFGF和TH基因的表达受PKC调控，Müller细胞可能为近视信号因子的一个重要来源。

英文摘要：

Objective Present paper was to investigate the effects of Protein kinase C （ PKC ） on tyrosine hydroxylase （ TH ）, inducible nitric oxide synthase （ iNOS ）, neuronal nitric oxide synthase （ nNOS ）, basic fibroblast growth factor （ bFGF）, transforming growth factor-β（TGFβ） in retinal Mailer ceils of the guinea pig myopic eye. Methods The myopia model was induced in the right eyes of 20 3-week-old guinea pigs by translucent goggles for 10 days, and 10 normal guinea pigs were as control. Retinal Mailer cells from the model and control pigs were saperated and cultured using enzyme-digesting method in DMEM containing 20% fetal bovine serum. The Muller cells were identified by immunocytochemistry. According to different intervening factors, retinal Mailer cells were divided into normal control,myopia group,myopia plus GFlO9203X group,myopia plus phorbol-12-myristate-13-acetate （PMA） group and myopia plus DMSO group. The mRNA expression of TH, iNOS, nNOS, bFGF and TGF[3 in retinal Müller cells were analyzed by real-time polymerase chain reaction （RT-PCR）. Results The ocular axle was elongated and the diopter was （ -3. 12±0. 50）D in occluded eyes ,showing statistically significant difference in comparison with control eyes（P 〈 0. 05）. The over 95% cultured cells were showed the positive reaction for GFAP. Compared with normal control group,the expression of mRNA of iNOS,nNOS,bFGF and TGFβ was significantly up-regulated in retinal Mtiller cells but down-regulated in TH mRNA expression in the myopic eyes （ P 〈 0. 05 ）. The expression of nNOS ,iNOS ,TH and bFGF was increased in cultured Maller cells in myopia plus PMA group compared with control group （P 〈0. 05）. However,the PKC activity was significantly reduced,and the mRNA of nNOS,iNOS,TH and bFGF was down-regulated in cultured ceils in myopia plus GF109203X group （ P 〈 0. 05 ）. Conclusion The gene expression of nNOS, iNOS, bFGF and TH is modulated by PKC in retinal Mailer cells in myopia of guinea pig. It