以大豆杂交组合皖82—178×通山薄皮黄豆甲衍生的重组自交系群体(RIL)为材料,以该群体所构建的遗传连锁图谱为基础,以2004和2005年油份含量为指标,利用软件Cartgrapher(V.2.0)采用复合区间作图法进行了QTL分析,结果表明,利用两年资料对油份含量QTL的定位结果基本一致。两年资料所检测到的QTL均位于wt-11连锁群的satt331附近,分别可以解释13.95%和15.01%遗传变异。此外,利用软件QTL Mapper1.6,采用复合区间作图法直接对两年的油份含量进行QTL联合分析,结果表明,控制油份的QTL也位于wt-11连锁群的satt331附近。
The soybean Recombinant Inbred Lines(RILL including 133 lines, from the cross Wan82-178 × Tongshanbaopihuangdoujia were used as experimental materials in this study. Based on the linkage map constructed with Single Sequence Repeat(SSR) markers using this RIL population, the software Cartgrapher(V.2.0) and the composite interval mapping were employed to identify quantitative traits loci(QTL) associated with oil content of soybean in 2004 and 2005. It was found that the results of mapping QTL for the oil content were similar for these two years. They were both mapped near satt331 on linkage group wt-11, and they could be used to explain 13.95 % and 15.01% of the total variation of the oil content, respectively. In addition, the software QTL Mapper 1.6 was applied to detect QTLs related to oil content in two years. The result indicated that the QTL related to oil content was still mapped near satt331 on linkage group wt-11.