要组合NJ89-1不育株×南农73-935的F1、F1:2、F2:3的遗传数据表明在组合NJ89-1不育株×南农73-935中NJ89-1雄性不育性仍受一对隐性基因控制;随机选取组合NJ89-1不育株×南农73-935的一个F1:2株行群体作为分子标记定位群体,对NJ89-1不育基因进行SSR标记定位,结果发现Sat-402与NJ89-1不育基因连锁,参照Song等(2004)整合的大豆遗传连锁图谱,初步将NJ89-1不育基因定位于大豆C2遗传连锁群上,NJ89-1不育基因与Sat-402的遗传距离为9.6cM。
The analysis of genetic data of F1, F1:2 and F2:3 of cross NJ89- 1 sterile plant × Nannong73 (935 showed that the male sterility of NJ89-1 was still controlled by a single recessive nuclear gene. One population of a F2 line of cross NJ89-1 sterile plant X Nannong73(935 was selected randomly to map the male sterile gene of NJ89-1 through SSR markers. The results showed that Sat-402 was linked to the male sterile gene of NJ89-1. According to the integrated soybean genetic linkage groups of Song et al. (2004), it was found that Sat-402 was on the linkage group C2. Thus the male sterile gene of NJ89- 1 was preliminarily located on the linkage group C2. The linkage distance between Sat-402 and the male sterile gene of NJ89-1 was 9.6cM.