中文摘要：

目的探索水凝胶为载体包裹甲状旁腺细胞的可行性。方法复苏冻存的成人甲状旁腺细胞并培养，待细胞足量后，消化、离心、吹打得单细胞悬液，将壳聚糖溶液、交联剂、细胞悬液按比例混合制得水凝胶，加培养液后于37℃恒温箱中培养，隔13留取培养液上清检测甲状旁腺激素含量，并对凝胶内细胞染色观察细胞状态。结果成功复苏并培养成人甲状旁腺细胞，细胞贴壁生长呈梭形；水凝胶成胶时间〈1min；荧光扫描结果显示24h后胶内活细胞比例〉90％，连续观察3d，比例没有发生明显改变；培养液中均检测到甲状旁腺激素。结论水凝胶化的成人甲状旁腺细胞操作简单，甲状旁腺细胞被水凝胶包裹后，不仅能够维持细胞的正常形态，且能够稳定释放甲状旁腺激素，有成为细胞载体并应用于移植治疗甲状旁腺功能减退症的可能。

英文摘要：

Objective To explore the feasibility of the hydrogel as a cell carrier for parathyroid cells. Methods Cryopreserved parathyroid cells were recovered and cultured until sufficient, then the cells were digested,centrifuged and pipetted to single-cell suspension. The chitosan solution, crosslinking agents and cell suspension were mixed to hydrogels by some ratio. After that, hydrogels covered by complete medium were incubated at 37 ℃ in constant temperature incubator. Meanwhile, the supernatant were collected for measuring the PTH level every other day and the viability and shape of cells in hydrogels was also evaluated by fluorescopy. Results Parathyroid cells were recovered and cultured successfully and the adherent cells were spindle-like. The hydrogel could be prepared at room temperature in less than 1 minutes. The fluorescent confocal microscopy showed that more than 90% of the cells in the hydrogel kept viable after 24 hours and the proportion had no significant change after 3 days. Furthermore, PTH was detected in the supernatants. Conclusion The facilely prepared inexpensive hydrogel, which can maintain normal morphlology and function of cells, is of potential application to cell transplantation therapy for hypopatathyroidism.