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中文摘要：
采用传统染色与克隆测序的方法,研究了8年不同施肥（氮磷）梯度对垂穗披碱草根系中丛枝菌根（AM）侵染率和AM真菌群落的影响.结果表明：随施肥浓度升高,垂穗披碱草根系单位根长AM总侵染率从67.5%下降至7.3%,丛枝侵染率从5.2%降至0.1%.根系共检测出24个AM真菌分子种,但随着施肥浓度上升,AM真菌的平均物种丰富度从6种下降至2.6种.不同施肥处理对AM真菌群落结构有显著影响,土壤速效磷和根系氮含量与AM真菌群落呈极显著相关.氮磷有效性随施肥梯度逐渐上升,且与AM侵染率和AM真菌物种丰富度呈显著负相关.施高浓度氮磷肥对AM共生体有明显的抑制作用,导致AM真菌物种多样性丧失.
英文摘要：
By using traditional staining and DNA cloning and sequencing techniques,this paper studied the effects of fertilization on the arbuscular mycorrhizal（AM） colonization and AM fungal community in Elymus nutans roots across an 8-year nitrogen（N） and phosphorus（P） fertilization gradient.With the increasing level of fertilization,the AM colonization and arbuscular colonization per unit root length reduced from 67.5% to 7.3% and from 5.2% to 0.1%,respectively.A total of 24 AM fungal phylotypes were detected in E.nutans roots,whilst the mean AM fungal richness reduced from 6 to 2.6 across the fertilization gradient.Different fertilization had significant effects on the AM fungal community composition,and there existed significant correlations between the AM fungal community composition and the soil available P and root N contents.The availability of N and P increased gradually across the fertilization gradient,and had significant negative correlations with both AM colonization and AM fungal richness.It was suggested that high level fertilization would reduce the activity of AM symbionts,and result in the large loss of AM fungal biodiversity.
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