中文摘要：

目的研究股神经肌支和隐神经纤维损伤7 d后二者蛋白表达的差异。方法成年雌性SD大鼠20只,体质量220～250 g。将大鼠右侧股神经干横断,左侧作为对照组不作处理。7 d后分别对正常股神经肌支及隐神经、损伤股神经肌支及隐神经取材,运用同位素标记相对和绝对定量技术标记（114标记正常的股神经肌支;115标记损伤的股神经肌支;116标记正常的隐神经;117标记损伤的隐神经）并结合二维液相色谱-串联质谱技术进行差异蛋白分析。结果共鉴定出3 358个蛋白。其中损伤的股神经肌支与正常的股神经肌支相比差异蛋白293个,表达升高112个,表达降低181个;损伤的隐神经神经与正常的隐神经相比差异蛋白417个,表达升高184个,表达降低233个。结论成功筛选出大鼠股神经运动神经纤维与感觉神经纤维损伤前后的差异蛋白,可为周围神经再生研究提供基础数据。

英文摘要：

Objective To identify the protein profile of rami musculares nervi femoralis and saphenous nerve in rat at 7th day after transection. Methods Total of 20 female adult SD rats,weighing 220-250 g,were included in this study.The right femoral nerve trunk transection was performed to rats,and the left side served as the control group. Seven days later,samples of normal/injured rami musculares nervi femoralis and saphenous nerve were obtained and labeled using i TRAQ（114,115,116,117 were used for labeling normal rami musculares nervi femoralis,injured rami musculares nervi femoralis,normal saphenous nerve,injured saphenous nerve,respectively）,and analyzed the data using 2DLC-MS/MS. Results A total of 3 358 proteins were identified.There are 293 differentially expressed proteins in the injured rami musculares nervi femoralis compared to the normal rami musculares nervi femoralis,in which 112 were up-regulated and 181 were down-regulated. There are 417 differentially expressed proteins in the injured saphenous nerve compared to the normal group,in which 184 were up-regulated and 233 were down-regulated.Conclusion We screen out differentially expressed proteins of rami musculares nervi femoralis and saphenous nerve in the process of Wallerian degeneration,which may contribute to the further study in peripheral nerve regeneration.