中文摘要：

在细菌纤维素纳米纤维网络结构中采用吐伦试剂与含醛基化合物原位反应生成纳米银颗粒，制备了纳米银／细菌纤维素（n-Ag／BC）复合凝胶膜，研究了不同反应条件对复合材料的银含量、化学结构和晶体结构的影响以及n-Ag／BC的微观结构和纳米银在纤维素网络中的存在形态；探讨了纳米银颗粒在纤维素网络中的形成机理；采用伤口常见细菌之一金黄色葡萄球菌测试了n-Ag/BC的抑菌性能；将n-Ag/BC与胎鼠表皮细胞共培养考察了材料的生物相容性．研究结果表明，在细菌纤维素纳米网络结构中可生成直径约为几十纳米的单质纳米银粒子；n-Ag／BC的银含量随着吐伦试剂浓度的增加而增加，同时银含量还取决于含醛基化合物的用量；原位反应生成纳米银粒子后细菌纤维素的晶型和结晶度没有发生变化；纳米银颗粒在细菌纤维素纳米网络结构的交叉处生成，复合材料n-Ag／BC对金黄色葡萄球菌的抑菌率达到99％以上，不影响细胞的增殖和分化过程，具有良好的生物相容性，是一种有广阔应用前景的创伤修复抗感染材料．

英文摘要：

Silver nanoparticles/bacterial cellulose （n-Ag/BC composite membranes were prepared and cha- racterized. Silver nanoparticles were synthesized in situ through the reaction of Tollens＇ reagent with alde- hydes, under ambient conditions in nanoporous bacterial cellulose membrances as nanoreactors. Sliver nanop- article was readily obtained and grew into the BC network by the precipitation of above reaction products. BC films were prepared and loaded with ca. 0. 14 mg/cm^2 to ca. O. 42 mg/cm^2 of silver nanopartieles. XRD pat- terns indicated the existence of Ago nanoparticles in the BC, and the diameter of the silver nanoparticles is ca. 31.8 nm. Scanning electron microscopy（SEM） images showed that the sliver nanoparticles（size range dozens nm） well dispersed in the network of BC. The sliver nanoparticle-impregnated BC membranes exhibited high hydrophilic ability and strong antimicrobial activity. Bacterial killing efficieneies of the silver loaded films were investigated against staphylococcus aureus. It was determined that as little as ca. 0. 14 rag/era2 of silver in the BC films caused a reduction of 99% bacteria in suspensions incubated in contact with the films. Significantly, the n-Ag/BC antimicrobial membranes were good biocompatibility, and allowed the attachment and growth of the epidermal cells. The result shows an easy method to synthesis silver nanoparticles into BC membranes. The n-Ag/BC has stability, antimicrobial activity and biological properties. The preparative procedure is facile, and provides a simple route to manufacturing of useful antimicrobial membranes, which would be a good alternative for antimicrobial wound dressing.