中文摘要：

目的：观察人I型磷脂酶A2（PLA2）的N末端衍生多肽PLA2N1-15对不同细菌的体外杀菌活性。方法：以人I型PLA2N末端的15个氨基酸残基为模板，合成多肽PLA2N1-15.采用琼脂铺板计数法，记录并计算不同质量浓度（1000、250、62．5、15．625μg／ml）的PLA2N1-15对革兰阳性（G^＋）菌（金黄色葡萄球菌、粪肠球菌、枯草芽孢杆菌）和革兰阴性（G^-）菌（大肠埃希菌、铜绿假单胞菌、变形杆菌）的杀菌率。结果：15．625、62．5、250、1000gg／ml的PLA2N1-15对G^＋菌的杀菌率分别为10％～19％、36．55％～89．73％、83％～100％、96％～100％，对G^-菌的杀菌率分别为8．86％～22．63％、21．61％～40．55％、40％～83％、87％～93％。结论：高浓度PLA2N1-15对G^＋和G^-均具有较强的杀菌活性，特别是对G^＋。

英文摘要：

OBJECTIVE： To observe the bactericidal activity of the polypeptide derived from N-terminal sequence of human type I phospholipase A2 （PLA1N1-15） in vitro. METHODS： PLA2N1-15 was synthesized according to 15 amino acid sequence from N-terminal of human type I PLA2. The sterilizing rate of different concentrations of PLA2N1-15 （1 000, 250, 62.5, 15.625 μg/ml） to Gram-positive bacteria （including Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis） and Gram-negative bacteria （including Escherichia coli, Pseudomonas aeruginosa, Proteusbacillus vulgaris） were calculated and recorded by agar plate colony counting. RESULTS： The sterilizing rates of 15.625, 62.5, 250 and 1 000 μg/ml PLA2N1-15 to Gram-positive bacteria were 10%- 19%, 36.55%-89.73%, 83%-100% and 96%-100%, respectively; those to Gram-negative bacteria were 8.86%-22.63%, 21.61%- 40.55 %, 40 % -83 % and 87 %-93 %, respectively. CONCLUSIONS： High concentration of PLA2N1-15 possesses strong bactericidal activity to Gram-positive bacteria and Gram-negative bacteria, particular to Gram-positive bacteria.