中文摘要：

目的探讨自发性高血压（SH）大鼠外周血CD_4＋T淋巴细胞和CD_8＋T淋巴细胞连接蛋白（Cx）43与炎性因子白介素（IL）-2、IL-6表达水平的相关性。方法于2015年4月—2016年4月,选取SH大鼠和正常血压的Wistar京都（WKY）大鼠各20只。采集腹主动脉外周血,血浆用于酶联免疫吸附试验（ELISA）法检测IL-2和IL-6表达水平;下层血细胞用于分离外周血淋巴细胞。收集5×10^5个淋巴细胞,采用流式细胞仪检测CD_4＋T淋巴细胞和CD_8＋T淋巴细胞Cx43阳性表达率。将淋巴细胞分为空白对照组、刀豆蛋白A（Con A）组（培养48 h后加入5μg/ml Con A）和Gap27＋Con A组（培养前加入500μmol/L Gap27,培养48 h后加入5μg/ml Con A）。提取淋巴细胞RNA,采用实时荧光定量RCR法检测各组淋巴细胞IL-2 mRNA与IL-6 mRNA相对表达水平。结果 SH大鼠IL-2表达水平为（152.40±29.62）pg/ml,高于WKY大鼠的（73.41±20.16）pg/ml（t=7.24,P〈0.05）;SH大鼠IL-6表达水平为（29.74±1.90）pg/ml,高于WKY大鼠的（22.58±1.06）pg/ml（t=14.72,P〈0.05）。SH大鼠CD_4＋T淋巴细胞Cx43阳性表达率为（3.48±0.58）%,高于WKY大鼠的（1.36±0.18）%（t=15.61,P〈0.05）。SH大鼠外周血CD_8＋T淋巴细胞Cx43阳性表达率为（39.02±4.77）%,高于WKY大鼠的（27.48±1.90）%（t=10.05,P〈0.05）。SH大鼠各组淋巴细胞IL-2 mRNA、IL-6 mRNA表达水平比较,差异均有统计学意义（P〈0.05）,其中Con A组IL-2mRNA、IL-6 mRNA表达水平均高于空白对照组和Gap27＋Con A组,Gap27＋Con A组IL-6 mRNA表达水平低于空白对照组（P〈0.05）。WKY大鼠各组淋巴细胞IL-2 mRNA表达水平比较,差异有统计学意义（P〈0.05）,其中Con A组IL-2 mRNA表达水平高于空白对照组和Gap27＋Con A组（P〈0.05）。结论 SH大鼠伴随炎性反应,炎性因子释放增加,应用缝隙连接阻断剂阻断淋巴细胞间信息通讯后,炎性因子的释放显著下降,缝隙连接可能参与高血压的炎性?

英文摘要：

Objective To analyze the relationship between Cx43 in CD_4＋and CD_8＋T cells in peripheral blood and levels of IL-2 and IL-6 among spontaneously hypertensive（ SH） rats.Methods During April 2015 to April 2016,20 rats with SH rats and 20 Wistar Kyoto（ WKY） rats with normal arterial pressure were selected as experimental animals.The peripheral blood from aorta abdominalis was collected and centrifuged,the plasma was used to detect levels of IL-2 and IL-6 by using ELISA method,the lower blood cells were used to separate peripheral blood lymphocytes.A total of 5 × 10~5 lymphocytes were collected,the positive expression rate of Cx43 in CD_4＋ and CD_8＋T cells was detected by the flow cytometer.The lymphocytes were divided into blank control group,Concanavalin A（ Con A） group（ 5 μg / ml Con A was added after 48 hours of culture）,and Gap27 ＋Con A group（ 500 μmol / L Gap27 was added before culture,5 μg / ml Con A was added after 48 hours of culture）.RNA was extracted from lymphocytes,the expression levels of IL-2 mRNA and IL-6 mRNA were detected by real-time RT-PCR.Results The expression level of IL-2 〔（ 152.40 ± 29.62） pg / ml〕among SH rats was significantly higher than that 〔（ 73.41± 20.16） pg / ml〕among WKY rats（ t = 7.24,P〈0.05）,the expression level of IL-6 〔（ 29.74 ± 1.90） pg / ml〕among SH rats was significantly higher than that 〔（ 22.58 ± 1.06） pg / ml 〕among WKY rats（ t = 14.72,P〈0.05）.The positive expression rate of Cx43 in CD_4＋T cells 〔（ 3.48 ± 0.58） % 〕among SH rats was significantly higher than that 〔（ 1.36 ± 0.18） % 〕among WKY rats（ t = 15.61,P〈0.05）,the positive expression rate of Cx43 in CD_8＋T cells 〔（ 39.02 ± 4.77） % 〕among SH rats was significantly higher than that 〔（ 27.48 ± 1.90） % 〕among WKY rats（ t = 10.05,P〈0.05）.For SH rats,there were significant differences in expression levels of IL-2 mRNA and IL-6 mRNA among different groups（ P〈0.05）,the expression levels of I