中文摘要：

为探讨2-APB对Wistar大鼠脑微动脉平滑肌细胞间缝隙连接的作用。采用全细胞膜片钳技术,观察2-APB对Wistar大鼠脑微动脉段上平滑肌细胞膜电容（Cinput）、膜电导（Ginput）和膜电阻（Rinput）的影响。结果显示,应用2-APB后Wistar大鼠脑动脉段平滑肌细胞的Cinput、Ginput减小或Rinput增大。当2-APB浓度≥100μmol/L时Wistar大鼠脑微动脉段上平滑肌细胞的Cinput、Ginput分别从81±18 pF和3.5±0.43 nS降至10.5±1.3pF（n=7,P〈0.01）和0.35±0.03nS（n=7,P〈0.01）,这与单个平滑肌细胞十分接近。由此可知,2-APB可以浓度依赖性的抑制Wistar大鼠脑微动脉平滑肌细胞间缝隙连接。

英文摘要：

To investigate the role of 2-APB gap junction between the smooth muscle cells of cerebral arteriole in Wistar rat. Whole-cell patch clamp recording was performed to study the effects of 2-APB on the whole-cell membrane input capacitance（Cinput）,membrane input conductance（Ginput） and membrane input resistance（Rinput） of vascular smooth muscle cell（VSMC） embedded in cerebral arteriole segment in Wistar rat. The result showed that application of 2-APB decreased the Cinput, Ginput,or increased Rinputof VSMC in cerebral arteriole segment in Wistar rat.When the concentration of 2-APB ≧ 100 μmol/L,the Cinputand Ginputreduced from 81±18 pF and 3.5±0.43 nS to10.5±1.3 pF（n=7,P〈0.01） and 0.35± 0.03nS（n=7,P〈0.01） respectively,which was very close to that of the single VSMC in Wistar rat. It was proved that 2-APB inhibits gap junctions between VSMCs in a concentration-dependent manner in cerebral arteriole in Wistar rat.