中文摘要：

目的 研究T淋巴细胞亚群在不同周龄的自发性高血压大鼠（spontaneously hypertensive rat,SHR）和正常Wistar京都（Wistar-Kyoto,WKY）大鼠脾脏中的表达差异。方法 选取16周龄和40周龄雄性SHR和WKY大鼠各8只,监测血压后,通过苏木素-伊红（HE）染色观察大鼠脾脏病理学变化,应用流式细胞术检测WKY大鼠和SHR脾脏中T淋巴细胞亚群的表达变化。结果 与相同周龄WKY大鼠比较,SHR脾脏中央动脉血管壁增厚,管腔狭窄,且40周龄SHR脾脏病变较16周龄严重;相同周龄的SHR与WKY大鼠比较,脾脏中CD3＋、CD3＋CD4＋和CD3＋CD8＋T淋巴细胞比例均无统计学差异（P〉0.05）;与16周龄WKY大鼠比较,40周龄WKY大鼠脾脏CD3＋和CD3＋CD8＋T淋巴细胞比例显著降低（P〈0.01）,CD3＋CD4＋T淋巴细胞比例和CD4＋/CD8＋比值显著升高（P〈0.01）;40周龄SHR脾脏CD3＋、CD3＋CD4＋、CD3＋CD8＋T淋巴细胞以及CD4＋/CD8＋比值的变化趋势同WKY大鼠相一致。结论 高血压可引起脾脏病理改变,但其对脾脏T淋巴细胞亚群比例可能无显著影响,然而随着周龄的增加,脾脏T淋巴细胞亚群均发生紊乱。

英文摘要：

Objective To investigate the difference in the expression of T lymphocyte subsets in the spleen of spontaneously hypertensive rats （SHRs） and Wistar-Kyoto （WKY） rats at different weeks of ages. Methods Sixteen-week-old and forty-week-old male SHRs and WKY rats （n=8 respectively） were used in this study. After measurement of blood pressure, the pathological changes in spleen tissue were observed by hematoxylin-eosin （HE） staining. And splenic T lymphocyte subsets of WKY rats and SHRs were detected by flow cytometry. Results Compared with age-matched WKY rats, SHRs showed thickening of central artery vascular walls and luminal stenosis; spleen pathology was more serious in forty-week-old SHRs than in sixteen-week-old SHRs. No significant differences were observed in the percentage of CD3＋, CD3＋CD4＋ or CD3＋CD8＋ T lymphocytes in the spleen of age-matched SHRs and WKY rats （P〉0.05）. Compared with sixteen-week-old WKY rats, forty-week-old WKY rats had a significantly decreased percentage of CD3＋ and CD3＋CD8＋ T lymphocytes in the spleen （P〈0.01）, but an increased percentage of CD3＋CD4＋ T lymphocytes and CD4＋/CD8＋ ratio in the spleen （P〈0.01）. The change tendency for CD3＋, CD3＋CD4＋ and CD3＋CD8＋ T lymphocytes and CD4＋/CD8＋ ratio in forty-week-old SHRs was consistent with that in the spleen of WKY rats. Conclusion Hypertension can cause pathological changes of the spleen, but it may not significantly affect splenic T lymphocyte subsets. However, aging may disrupt the homeostasis of T lymphocyte subsets.