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中文摘要：
通过单因素实验方法研究发酵液初始pH值、NH4^＋、Fe^2＋、Mg^2＋浓度以及厌氧活性污泥的热处理条件对厌氧发酵产氢的影响。归纳得到最适宜产氢条件为：厌氧活性污泥在90℃下热处理40min，调节发酵液初始pH值至8．0并添加3．00，0．10和0．03g／L的（NH4）2SO4，FeSO4和MgSO4。在该最适宜条件下得到较高纯度（体积分数为0．80）的氢气630mL，1mol葡萄糖可产氢气2．62mol。实验表明厌氧发酵是较为高效的产氢方式。
英文摘要：
Anaerobic dark fermentation experiments were performed in microcosms to produce biohydrogen from glucose. The influences of pH, concentration of NH4^＋ , Fe^2＋ and Mg^2＋ , and the temperature and duration of preheating on the hydrogen production efficiency were evaluated via single factor experiments. The optimal conditions were concluded to be preheating for 40 min at 90 ℃ followed by dark fermentation at pH 8.0 with 3.00, 0. 10 and 0.03 g. L^-1 of （NH4 ）2SO4, FeSO4 and MgSO4, respectively. Under these conditions, 630 mL high quality H2 （ purity： 0.80） was harvested cumulatively with a yield of 2.62 mol H2 per mol glucose. The overall results suggest that anaerobic dark fermentation is an effective approach for biohydrogen generation.
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