中文摘要：

以蛋白质或多肽修饰的吲哚类菁染料Cy3为内核，采用实验条件简单的油包水反相微乳液方法成核，通过正硅酸乙酯水解形成的网状二氧化硅包壳的方法制备吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒．考察了以不同等电点的蛋白质和多肽修饰的Cy3为内核材料对吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒制备的影响．结果表明，分别采用人免疫球蛋白（IgG）或多聚赖氨酸修饰的Cy3为内核材料，都能制备荧光强度高、荧光稳定性强和染料泄漏极少的Cy3嵌入的核壳荧光纳米颗粒．进一步对Cy3嵌入的核壳荧光纳米颗粒进行了表征，并将基于这一新型的荧光纳米颗粒建立起来的生物标记方法初步应用于流感病毒DNA的检测，其检测线性范围为3．18×10^-10～1．27×10^-9mol／L，检测下限为3．51×10^10 mol／L，相关系数r为0．9865．

英文摘要：

Cy3 dye doped core-shell silica fluorescent nanoparticles was synthesized by using of water-in-oil microemulsion technique, with biomolecules modified Cy3 as the core and the silica produced from the hydrolysis TEOS（tetraethyl orthosilicate） as the shell. In this paper, five kinds of bimolecules with different pl values for preparation of Cy3 doped core-shell silica fluorescent nanoparticles were investigated. The results show that very bright and photostable Cy3 doped core-shell silica fluorescent nanoparticles can be both prepared by using Cy3-IgG or Cy3-polysine modified as the core material. And then Cy3-IgG was selected as a demonstration to prepare Cy3 doped core-shell silica fluorescent nanoparticles. A novel fluorescence marker was based by using the Cy3 doped core-shell silica fluorescent nanoparticles. The flu virus DNA was detected by using the Cy3 doped core-shell silica fluorescent nanoparticles, and the detection limit of the target DNA is 3.51 × 10^-10 mol/L.