Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon
- ISSN号:1000-0593
- 期刊名称:《光谱学与光谱分析》
- 时间:0
- 分类:Q811.4[生物学—生物工程]
- 作者机构:[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China, [2]Life Science and Biotechnology Institute, Hunan University, Changsha 410082, China, [3]Chemistry and Chemical Engineer College, Hunan University,Changsha 410082, China, [4]Hunan Engineering Research Center for Bio-Nanotechnology, HunanUniversity, Changsha 410082, China, [5]Medical Experiment Research Center, Xiangya Hospital, CentralSouth University, Changsha 410008, China, [6]Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Changsha 410008, China
- 相关基金:Acknowledgements This work was supported by the National Key Basic Research Program of China (Grant Nos. 2002CB 513110 and 2004CB520804); the Key Technologies Research and Development Program (Grant No. 2003 BA310A 16); the International Key Corporation Project of the Chinese Technology Ministry (Grant No. 2003DF000039); the National Natural Science Foundation of China (Grant No. 20135010); the Key Project of Natural Science Foundation of Hunan Province (Grant No. 0399Y1006).
作者:
LIU Bin[1,2,3,4] WANG Kemin[1,2,3,4] XIAO Zhiqiang[5,6] WANG Wei[1,3,4] TAN Weihong[1,2,3,4] SUN Yi[5,6] TANG Hongxing[1,3,4] YANG Xiaohai[1,3,4]
中文摘要:
药处理,基因 transfection,和在肿瘤房间的 ING1 mRNA 的水平上的 p53 RNA 干扰(RNAi ) 的效果是在 ING1 分子的烽火(MB ) 的帮助下检测的份量上 /cRNA 标准曲线基于 MB 察觉技术。结果证明 mRNA 能是起来的 ING1 的水平在 MCF-7 的低表示房间线由 5-FU 处理或 ING1 transfection 调整了并且被 silencing p53 在 CNE2 的正常表示房间线与 RNAi 技术禁止。ING1 的水平从 7.7 X 10 ~ 变化了(-16) 到 53.4 X 10 ~( 肿瘤的 -16) 摩尔 / 亩 g 总数 RNA 装壁板圩。结果不仅为基因表示的规定效果提供了证据而且被用于调查相互作用多信号 transduc-tionpathway 上的基因。
英文摘要:
The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53 with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10^-16 to 53.4× 10^-16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigating the interaction of multigenes on signal transduction pathway.